Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines
Cell Line Data Base: structure and recent improvements towards molecular authentication of human cell lines
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DOI：
10.1093/nar/gkn730
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发表时间：
2009-01-01
影响因子：
14.9
通讯作者：
Parodi, Barbara
中科院分区：
文献类型：
--
作者：
Romano, Paolo;Manniello, Assunta;Parodi, Barbara
细胞系数据库 (CLDB) 是有关人类和动物细胞系的著名参考信息源，包括 6000 多种细胞系的信息。主要生物学特征根据来自国际列表和分类法的受控词汇进行编码。 HyperCLDB (http://bioinformatics.istge.it/hypercldb/) 是 CLDB 的超文本版本，它还允许通过网络蜘蛛检索信息，从而提高数据可访问性。通过生物特征索引提供对 HyperCLDB 的访问，并且通过许多内部链接授予超文本中的导航。 HyperCLDB 还包含外部资源的链接。最近，人们对细胞系的参考命名法产生了兴趣，CLDB 被视为一个权威系统。此外，为了克服细胞系错误识别问题，提出了分子鉴定方法，例如指纹识别、单基因座短串联重复（STR）谱和单核苷酸多态性验证。由于这些数据是分布式的，因此需要一个关于人类细胞系认证的参考门户。我们在此介绍 CLDB 的架构和内容、其最新的增强功能和前景。我们还提出了一个新的相关数据库，细胞系集成分子认证（CLIMA）数据库（http://bioinformatics.istge.it/clima/），它允许将认证数据链接到实际的细胞系。
The Cell Line Data Base (CLDB) is a well-known reference information source on human and animal cell lines including information on more than 6000 cell lines. Main biological features are coded according to controlled vocabularies derived from international lists and taxonomies. HyperCLDB (http://bioinformatics.istge.it/hypercldb/) is a hypertext version of CLDB that improves data accessibility by also allowing information retrieval through web spiders. Access to HyperCLDB is provided through indexes of biological characteristics and navigation in the hypertext is granted by many internal links. HyperCLDB also includes links to external resources. Recently, an interest was raised for a reference nomenclature for cell lines and CLDB was seen as an authoritative system. Furthermore, to overcome the cell line misidentification problem, molecular authentication methods, such as fingerprinting, single-locus short tandem repeat (STR) profile and single nucleotide polymorphisms validation, were proposed. Since this data is distributed, a reference portal on authentication of human cell lines is needed. We present here the architecture and contents of CLDB, its recent enhancements and perspectives. We also present a new related database, the Cell Line Integrated Molecular Authentication (CLIMA) database ( http://bioinformatics.istge.it/clima/), that allows to link authentication data to actual cell lines.