In Vitro Kinetic Analysis of Fermentation of Prebiotic Inulin-Type Fructans by Bifidobacterium Species Reveals Four Different Phenotypes
In Vitro Kinetic Analysis of Fermentation of Prebiotic Inulin-Type Fructans by Bifidobacterium Species Reveals Four Different Phenotypes
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DOI：
10.1128/aem.01488-08
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发表时间：
2009-01-15
影响因子：
4.4
通讯作者：
De Vuyst, Luc
中科院分区：
文献类型：
--
作者：
Falony, Gwen;Lazidou, Katerina;De Vuyst, Luc
对生长在果糖、低聚果糖或菊粉上的18株双歧杆菌进行了细菌生长、碳水化合物消耗和代谢产物产生的动力学分析。一个主成分分析的数据集，扩大与有关的β-呋喃果糖苷酶基因的存在下，以前遇到的动物双歧杆菌亚种的遗传筛选的结果。lactis DSM 10140（T），揭示了在所测试的双歧杆菌中存在四个簇。属于第一簇的菌株不能降解低聚果糖或菊粉。在第二个集群中的菌株可以降解低聚果糖，表现出优先的分解机制，但没有生长在菊粉上。果糖消耗快于低聚果糖降解。第三个集群是由菌株降解所有低聚果糖馏分同时，可以部分打破菊粉。低聚果糖的降解比果糖的消耗快得多。第四，较小的集群由共享高果糖消耗和低聚果糖降解率，并能够执行菊粉的部分分解的菌株。对于所有的菌株，代谢转向更多的乙酸盐，甲酸盐和乙醇生产，在乳酸盐生产的代价，观察到在生长过程中不易发酵的能源。不能检测到故障模式和β-呋喃果糖苷酶基因的存在之间的相关性。这些变化表明生态位特定的适应的双歧杆菌，并可能在体内的影响，对菌株特异性的菊粉型果聚糖的刺激作用的双歧杆菌。
Kinetic analyses of bacterial growth, carbohydrate consumption, and metabolite production of 18 Bifidobacterium strains grown on fructose, oligofructose, or inulin were performed. A principal component analysis of the data sets, expanded with the results of a genetic screen concerning the presence of a beta-fructofuranosidase gene previously encountered in Bifidobacterium animalis subsp. lactis DSM 10140(T), revealed the existence of four clusters among the bifidobacteria tested. Strains belonging to a first cluster could not degrade oligofructose or inulin. Strains in a second cluster could degrade oligofructose, displaying a preferential breakdown mechanism, but did not grow on inulin. Fructose consumption was faster than oligofructose degradation. A third cluster was composed of strains that degraded all oligofructose fractions simultaneously and could partially break down inulin. Oligofructose degradation was substantially faster than fructose consumption. A fourth, smaller cluster consisted of strains that shared high fructose consumption and oligofructose degradation rates and were able to perform partial breakdown of inulin. For all strains, a metabolic shift toward more acetate, formate, and ethanol production, at the expense of lactate production, was observed during growth on less readily fermentable energy sources. No correlation between breakdown patterns and the presence of the beta-fructofuranosidase gene could be detected. These variations indicate niche-specific adaptation of bifidobacteria and could have in vivo implications on the strain specificity of the stimulatory effect of inulin-type fructans on bifidobacteria.