Genetic transactivation of Dissociation elements in transgenic tomato plants
Genetic transactivation of Dissociation elements in transgenic tomato plants
复制标题
转基因番茄植物中解离元件的遗传反式激活
DOI：
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
J. Yoder
中科院分区：
文献类型：
--
作者：
M. Lassner;J. Palys;J. Yoder
我们以前报道过玉米转座因子激活子（Ac）和解离因子（Ds）在转基因番茄中的行为。为了继续我们对番茄Ac-Ds家族的研究，我们构建了两个Ds元件和一个稳定的Ac衍生物，命名为转座酶（Ts 101）。两个构建的Ds元件各自含有细菌β-半乳糖苷酶基因，以便于通过标记拯救从番茄基因组克隆元件。在Ds 202中，Ac的中心1.6kb HindIII片段被嵌合的细菌β-半乳糖苷酶基因取代。Ds 204含有来自pUC 19的β-半乳糖苷酶片段，两侧分别是185 bp和131 pb的Ac 5′和3′末端（相对于转录方向）。Ts 101元件是通过从Ac的3′端删除约50个核苷酸（包括反向重复）而构建的。Ts 101和两个Ds元件在转化番茄植株中稳定。将含有Ds 1的两个构建的Ds元件中的任一个的转基因植物与含有Ts 101或完整Ac元件的转基因植物杂交，Ds 1是先前从玉米Adh 1基因座克隆的元件。Southern杂交分析表明，Ds 1和Ds 202切除其居民T-DNA位置在所有的后代含有Ts 101或Ac，但稳定的兄弟姐妹没有遗传转座酶。虽然Southern分析没有发现转座Ds元件在F1代，F2群体的分析表明，Ds元件已重新整合在番茄基因组中。与这些结果相反，没有检测到Ds 204的Ac催化切除，即使该元件含有能够形成二级结构的Ac末端区域。我们已经证明，Ds元件可以在转基因番茄植物中通过与含有Ac或Ts元件的植物杂交而被反式激活。
SummaryWe have previously reported on the behaviour of the maize transposable elements Activator (Ac) and Dissociation (Ds) in transgenic tomato plants. To continue our study of the Ac-Ds family in tomato, we have constructed two Ds elements and a stable Ac derivative designated Transposase (Ts101). The two constructed Ds elements each contain a bacterial β-galactosidase gene to facilitate cloning the elements from the tomato genome by marker rescue. In Ds202, the central 1.6 kb HindIII fragment of Ac was replaced by a chimaeric bacterial β-galactosidase gene. Ds204 contains the β-galactosidase fragment from pUC19 flanked by 185 bp and 131 pb of the 5′ and 3′ termini (relative to the direction of transcription) of Ac. The Ts101 element was constructed by deleting about 50 nucleotides, including the inverted repeat, from the 3′ end of Ac. Ts101 and the two Ds elements were stable in transformed tomato plants. Transgenic plants containing either one of the two constructed Ds elements of Ds1, an element previously cloned from the maize Adh1 locus, were crossed to transgenic plants containing either Ts101 or an intact Ac element. Southern hybridization analysis showed that Ds1 and Ds202 excised from their resident T-DNA locations in all progeny which contained either Ts101 or Ac, but were stable in siblings which had not inherited a transposase. While Southern analysis did not identify transposed Ds elements in the F1 progeny, analysis of an F2 population indicated that the Ds elements had reintegrated in the tomato genome. In contrast to these results, no Ac-catalyzed excision of Ds204 was detected, even though the element contained the terminal regions of Ac capable of forming secondary structure. We have shown that Ds elements can be transactivated in transgenic tomato plants by crossing to plants containing an Ac or Ts element.
影响因子：
3.5
作者：
HENIKOFF, S
通讯作者：
HENIKOFF, S