The effects of dietary organic or inorganic selenium in rainbow trout (Oncorhynchus mykiss) under crowding conditions
The effects of dietary organic or inorganic selenium in rainbow trout (Oncorhynchus mykiss) under crowding conditions
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DOI：
10.1111/j.1365-2095.2008.00624.x
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发表时间：
2009-12-01
影响因子：
3.5
通讯作者：
Sahin, K.
中科院分区：
文献类型：
--
作者：
Kucukbay, F. Z.;Yazlak, H.;Sahin, K.
研究了不同硒源（亚硒酸钠或硒代蛋氨酸）对虹鳟鱼生长、血清氧化应激指标[丙二醛（MDA）、8-异前列腺素（8-isoprostane）、谷胱甘肽过氧化物酶（GSH-Px）活性]以及肌肉硒、丙二醛（MDA）和热休克蛋白70（Hsp 70）水平的影响。将初始平均体重为20 +/- 0.8 g的鱼（n = 360; 0 +岁）随机分配到12个处理组，每个处理组由3个重复10条鱼组成，每个重复10条鱼，采用2 x 2 x 3因子排列处理（放养密度、硒源、硒水平）。鱼被保持在低（25公斤m-3）或高（100公斤m-3）放养密度和喂养的基础（对照）饮食或补充有0.15或0.30毫克硒kg-1的饮食从两种不同的形式：亚硒酸钠或硒蛋氨酸的基础饮食。在基础日粮条件下，高饲养密度显著降低了增重、采食量和饲料转化率（P = 0.001）。线性增加摄食量和体重增加和改善FCR被发现在亚硒酸钠（P = 0.01）或硒代蛋氨酸（P = 0.001）补充鱼饲养拥挤的条件下。血清和肌肉硒水平和血清GSH-Px活性线性增加（P = 0.001），而血清和肌肉MDA浓度和血清8-异前列腺素线性下降，饲料中添加亚硒酸钠（P = 0.01）或硒蛋氨酸（P = 0.001）增加。添加硒蛋氨酸和亚硒酸钠显著降低了拥挤条件下鱼肌肉中Hsp 70的表达（P < 0.05）。补充硒可改善鱼的生长和抗氧化状态，在拥挤组中硒代蛋氨酸的效果相对大于亚硒酸钠。结果表明，拥挤的条件下造成显着的有害影响，虹鳟鱼表示增加氧化应激，减少摄食量和体重增加。这也表明，饲料硒的补充提供了一个可行的方法，减少在拥挤条件下饲养虹鳟鱼的性能损失。硒代蛋氨酸似乎比亚硒酸钠更有效，并且在本研究中高剂量似乎也比低剂量更有效。
In the present study, the effects of different sources of selenium (Se; sodium selenite or selenomethionine) supplementation on the growth and serum concentrations of oxidative stress markers [malondialdehyde (MDA), 8-isoprostane, glutathione peroxidase (GSH-Px) activity] and muscle Se, MDA and heat shock protein 70 (Hsp70) levels in rainbow trouts were evaluated. The fish (n = 360; 0 + years old) with initial average weight of 20 +/- 0.8 g were randomly assigned to 12 treatment groups consisting of 3 replicates of 10 fish each in a 2 x 2 x 3 factorial arrangement of treatments (stocking densities, Se sources, Se levels). The fish were kept at low (25 kg m-3) or high (100 kg m-3) stocking densities and fed a basal (control) diet or the basal diet supplemented with either 0.15 or 0.30 mg of Se kg-1 of diet from two different forms: sodium selenite or selenomethionine. High stocking density decreased weight gain, feed intake and feed conversion ratio (FCR) when basal diet was fed (P = 0.001). A linear increase in feed intake and weight gain and improvement in FCR were found in sodium selenite (P = 0.01)- or selenomethionine (P = 0.001)-supplemented fish reared under crowding conditions. Serum and muscle Se levels and serum GSH-Px activity increased (P = 0.001) linearly, whereas serum and muscle MDA concentrations and serum 8-isoprostane decreased linearly as dietary sodium selenite (P = 0.01) or selenomethionine (P = 0.001) supplementation increased. Selenomethionine and sodium selenite supplementation decreased Hsp70 in the muscle of fish reared under crowding conditions (P < 0.05). Supplementation with Se improved growth and antioxidant status of fish and the effects of selenomethionine were relatively greater than sodium selenite in the crowded groups. Results suggest that crowding conditions cause significant detrimental effects in rainbow trout indicated by increased oxidative stress, reduced feed intake and body weight gain. It also indicates that dietary Se supplementation offers a feasible way of reducing the losses in performance of rainbow trout reared under crowding conditions. Selenomethionine seems to be more effective than sodium selenite and the higer dose in the present study also seems to be more effective than the lower dose.