Temperature, pH and redox responsive cellulose based hydrogels for protein delivery
Temperature, pH and redox responsive cellulose based hydrogels for protein delivery
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DOI：
10.1016/j.ijbiomac.2016.02.042
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发表时间：
2016-06-01
影响因子：
8.2
通讯作者：
Dhara, Dibakar
中科院分区：
文献类型：
--
作者：
Dutta, Sujan;Samanta, Pousali;Dhara, Dibakar
基于纤维素的水凝胶由于其生物相容性、无毒和天然来源而重要。以羧甲基纤维素（CMC）和聚（N-异丙基丙烯酰胺）为原料，制备了一系列pH、温度和氧化还原响应性水凝胶。以N-异丙基丙烯酰胺（NIPA）和甲基丙烯酸羧甲基纤维素（CMC）为原料，合成了共聚型（CP）水凝胶，并在CMC存在下聚合了NIPA，制备了半互穿网络（SIPN）水凝胶。使用两种类型的交联剂，即N，N '-亚甲基双丙烯酰胺（BIS）和N，N'-双（丙烯酰基）胱胺（CBA），一种氧化还原敏感的交联剂。用FTIR和SEM对水凝胶的结构进行了表征。发现CP水凝胶比相应的SIPN更多孔，这导致CP水凝胶的更高溶胀。发现两种水凝胶的溶胀度均随着CMC含量的增加而增加。虽然SIPN水凝胶的溶胀表现出不连续的温度依赖性，但CP水凝胶的保水值随着温度的升高而逐渐降低。CBA交联的水凝胶相比于BIS交联的水凝胶显示出更高的溶胀。此外，溶菌酶负载在水凝胶中，并在不同的pH值，温度和还原剂，谷胱甘肽（GSH）的存在下，其在体外释放进行了研究。在较低的温度、较低的pH和GSH存在下，释放速率最大。(C)2016爱思唯尔B. V.保留所有权利。
Cellulose based hydrogels are important due to their biocompatibility, non-toxicity and natural origin. In this work, a new set of pH, temperature and redox responsive hydrogels were prepared from carboxymethylcellulose (CMC) and poly(N-isopropylacrylamide). Copolymeric (CP) hydrogels were synthesized by copolymerizing N-isopropylacrylamide (NIPA) and methacrylated carboxymethylcellulose, semi-interpenetrating network (SIPN) hydrogels were prepared by polymerizing NIPA in presence of CMC. Two types of cross-linkers were used viz. N,N'-methylenebisacrylamide (BIS) and N,N'-bis(acryloyl)cystamine (CBA), a redox sensitive cross-linker. The structures of the hydrogels were characterized by FTIR and SEM studies. The CP hydrogels were found to be more porous than corresponding SIPNs which resulted in higher swelling for the CP hydrogels. Swelling for both the hydrogels were found to increase with CMC content. While the swelling of SIPN hydrogels showed discontinuous temperature dependency, CP hydrogels showed gradual decrease in water retention values with increase in temperature. CBA cross-linked hydrogels showed higher swelling in comparison to BIS cross-linked hydrogels. Additionally, lysozyme was loaded in the hydrogels and its in vitro release was studied in various pH, temperature and in presence of a reducing agent, glutathione (GSH). The release rate was found to be maximum at lower temperature, lower pH and in presence of GSH. (C) 2016 Elsevier B.V. All rights reserved.