HUMAN PAPILLOMAVIRUS TYPES IMPORTANT IN PROGRESSION OF INVERTED PAPILLOMA
HUMAN PAPILLOMAVIRUS TYPES IMPORTANT IN PROGRESSION OF INVERTED PAPILLOMA
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DOI：
10.1016/s0194-5998(95)70046-3
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发表时间：
1995-11-01
影响因子：
3.4
通讯作者：
BRADFORD, CR
中科院分区：
文献类型：
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作者：
BECK, JC;MCCLATCHEY, KD;BRADFORD, CR
最近的证据表明，人乳头状瘤病毒可能在内翻性乳头状瘤的发病机制中发挥作用，内翻性乳头状瘤是一种良性但局部侵袭性的肿瘤，复发率高，与鳞状细胞癌相关。内翻性乳头状瘤的组织学特征在鉴别恶变高风险病变方面并不有用，我们研究了1980年至1994年在密歇根大学治疗的39例内翻性乳头状瘤患者的存档病理标本，使用聚合酶链反应技术和人乳头状瘤病毒L1和E6共有引物，以前我们曾报道，这些标本中有63%的人乳头瘤病毒序列检测呈阳性，并且人乳头瘤病毒的存在预示着内翻性乳头瘤的复发。我们使用型特异性引物对和聚合酶链反应技术，以及与型特异性寡核苷酸探针杂交，以确定人乳头瘤病毒的类型。高危型HPV感染与病变严重程度（异型增生或癌变）有显著相关性（P < 0.01）。所有12例含有人乳头瘤病毒的良性内翻性乳头瘤标本均检测为人乳头瘤病毒6型或11型阳性。在表现出异型增生的七个内翻性乳头状瘤标本中，五个是人乳头状瘤病毒阳性，三个含有人乳头状瘤病毒6，一个含有人乳头状瘤病毒11，一个含有人乳头状瘤病毒18，在含有与鳞状细胞癌相关的内翻性乳头状瘤的三个标本中的每一个中，标本的内翻性乳头状瘤部分对单一人乳头状瘤病毒类型检测为阳性：人乳头瘤病毒6、11或16型，在4例单独鳞状细胞癌的人乳头瘤病毒阳性标本中（患者先前在同一部位切除了内翻性乳头瘤），3例检测为人乳头瘤病毒16型阳性，1例未分型。
Recent evidence suggests that human papillomavirus may play a role in the pathogenesis of inverted papilloma, a benign but locally aggressive neoplasm with a high recurrence rate and an association with squamous cell carcinoma. Histologic features of inverted papilloma have not been useful in discriminating lesions at high risk for malignant transformation, We studied archival pathology specimens from 39 patients with inverted papilloma treated at the University of Michigan between 1980 and 1994 using polymerase chain reaction techniques and human papillomavirus L1 and E6 consensus primers, Previously we reported that 63% of these specimens tested positive for human papillomavirus sequences and that presence of human papillomavirus predicted recurrence of inverted papilloma. We used type-specific primer pairs and polymerase chain reaction techniques as well as hybridization with type-specific oligonucleotide probes to determine human papillomavirus type. A significant correlation was observed between the severity of the lesion (dysplasia or carcinoma) and high risk human papillomavirus type (p < 0.01). All 12 benign inverted papilloma specimens that contained human papillomavirus tested positive for human papillomavirus 6 or 11. Of seven inverted papilloma specimens that exhibited dysplasia, five were human papillomavirus positive, three contained human papillomavirus 6, one contained human papillomavirus 11, and one contained human papillomavirus 18, In each of the three specimens that contained inverted papilloma in association with squamous cell carcinoma, the inverted papilloma portion of the specimen tested positive for a single human papillomavirus type: human papillomavirus 6, 11, or 16, Of the four human papillomavirus-positive specimens with squamous cell carcinoma alone (patients who had an inverted papilloma previously resected at the same site), three tested positive for human papillomavirus 16, and 1 was untyped.