New Evidence for the Role of Ethylene in Strawberry Fruit Ripening
New Evidence for the Role of Ethylene in Strawberry Fruit Ripening
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DOI：
10.1007/s00344-012-9312-6
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发表时间：
2013-01
影响因子：
4.8
通讯作者：
Jing-hua Sun;Jing-jing Luo;L. Tian;Chun-li Li;Y. Xing;Yuan-Yue Shen
中科院分区：
文献类型：
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作者：
Jing-hua Sun;Jing-jing Luo;L. Tian;Chun-li Li;Y. Xing;Yuan-Yue Shen
有人认为植物激素乙烯在草莓果实成熟中发挥作用，本研究为该激素在草莓成熟中的作用提供了新的遗传学证据。对‘Camarosa’草莓（Fragariaananassa）果实中乙烯生物合成相关基因FaSAMS1和信号基因FaCTR1的乙烯产量和转录本的综合分析表明，在果实红色着色过程中，乙烯产量的增加伴随着这两个基因转录本的增加，表明FaSAMS1和FaCTR1可能在果实成熟中发挥作用。通过最近报道的烟草脆裂病毒诱导的基因沉默技术下调FaSAMS1或FaCTR1转录本不仅抑制了果实的红色和硬度，而且还促进了乙烯的生物合成。此外，后者还影响了一系列乙烯信号成分。重要的是，施用乙烯利可以促进天然草莓果实变红和软化，并部分挽救两种RNAi果实中花青素的生物合成，但不能显着影响RNAi果实的硬度。这些数据提供了新的证据，证明 FaCTR1 正向调节草莓果实成熟，并且乙烯是草莓果实成熟所必需的。
It has been suggested that the phytohormone ethylene plays a role in strawberry fruit ripening, and new genetic evidence for the role of this hormone in strawberry ripening is provided in this study. The combined analysis of ethylene production and transcripts of the ethylene biosynthesis-related geneFaSAMS1and the signaling geneFaCTR1in ‘Camarosa’ strawberry (Fragariaananassa) fruit showed that an increase in ethylene production was concomitant with a rise in transcripts of the two genes during fruit red-coloring, suggesting that FaSAMS1 and FaCTR1 might play a role in fruit ripening. Downregulation of theFaSAMS1orFaCTR1transcript via a recently reported tobacco rattle virus-induced gene-silencing technique not only inhibited fruit red-coloring and firmness, but it also promoted ethylene biosynthesis. Furthermore, the latter also affected a series of ethylene-signaling components. Importantly, applied ethephon could promote natural strawberry fruit red-coloring and softening and partially rescue anthocyanin biosynthesis in the two-type RNAi fruit, but could not markedly affect RNAi fruit firmness. These data provide new evidence that FaCTR1 positively regulates strawberry fruit ripening and that ethylene is required for strawberry fruit ripening.