A glucosyltransferase specific for 4-hydroxy-2,5-dimethyl-3(2H)-furanone in strawberry
A glucosyltransferase specific for 4-hydroxy-2,5-dimethyl-3(2H)-furanone in strawberry
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DOI：
10.1080/09168451.2018.1524706
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发表时间：
2019-01
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影响因子：
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通讯作者：
Akira Yamada;Kan’ichiro Ishiuchi;T. Makino;H. Mizukami;Kazuyoshi Terasaka
中科院分区：
文献类型：
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作者：
Akira Yamada;Kan’ichiro Ishiuchi;T. Makino;H. Mizukami;Kazuyoshi Terasaka
摘要4-羟基-2，5-二甲基-3（2 H）-呋喃酮（HDMF）是草莓中的一种重要香气成分。大量的HDMF转化为HDMF β-D-葡萄糖苷并在成熟草莓果实中积累。本研究从草莓中分离到一种新的UDP-葡萄糖：HDMF葡萄糖基转移酶UGT 85 K16。UGT 85 K16优先将HDMF及其结构类似物的羟基葡糖基化。虽然UGT 85 K16也催化香草醛的葡萄糖基化，但其对HDMF的亲和力和效率更高。UGT 85 K16 mRNA的表达与草莓中HDMF及其糖苷的积累相关。这些结果表明UGT 85 K16可能是草莓中UDP-葡萄糖：HDMF葡萄糖基转移酶。缩略语：DMMF：2，5-二甲基-4-甲氧基-3（2 H）-呋喃酮; EHMF：2（5）-乙基-4-羟基-5（2）-甲基-3（2 H）-呋喃酮; GBV：糖苷结合挥发物; HDMF：4-羟基-2，5-二甲基-3（2 H）-呋喃酮; HMF：4-羟基-5-甲基-3（2 H）-呋喃酮; HMMF：4-羟基-5-甲基-2-亚甲基-3（2 H）-呋喃酮; PSPG：植物次级产物糖基转移酶; RT-PCR：逆转录-PCR; OMT：O-甲基转移酶; UGT：UDP-糖基转移酶图形摘要草莓HDMF葡萄糖基转移酶（UGT 85 K16）的表征。
ABSTRACT 4-Hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is a key aroma compound in Fragaria × ananassa (strawberry). A considerable amount of HDMF is converted into HDMF β-D-glucoside and accumulated in mature strawberry fruits. Here we isolated a novel UDP-glucose: HDMF glucosyltransferase, UGT85K16 from Fragaria × ananassa. UGT85K16 preferentially glucosylated the hydroxyl group of HDMF and its structural analogs. Although UGT85K16 also catalyzed the glucosylation of vanillin, its affinity and efficiency toward HDMF was higher. The expression of UGT85K16 mRNA correlated with the accumulation of HDMF and its glucoside in Fragaria × ananassa plants. These results suggest that UGT85K16 might be UDP-glucose: HDMF glucosyltransferase in strawberries. Abbreviations: DMMF: 2,5-dimethyl-4-methoxy-3(2H)-furanone; EHMF: 2(5)-ethyl-4-hydroxy-5(2)-methyl-3(2H)-furanone; GBV: glycosidically bound volatile; HDMF: 4-hydroxy-2,5-dimethyl-3(2H)-furanone; HMF: 4-hydroxy-5-methyl-3(2H)-furanone; HMMF: 4-hydroxy-5-methyl-2-methylene-3(2H)-furanone; PSPG: Plant secondary product glycosyltransferase; RT-PCR: reverse transcription-PCR; OMT: O-methyltransferase; UGT: UDP-glycosyltransferase Graphical Abstract Characterization of HDMF glucosyltransferase (UGT85K16) from strawberry.