Genome sequence of the banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae) and its symbionts
Genome sequence of the banana aphid, Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae) and its symbionts
复制标题
香蕉蚜、Pentalonia nigronervosa Coquerel（半翅目：蚜科）及其共生体的基因组序列
DOI：
10.1101/2020.04.25.060517
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Mathers T
中科院分区：
文献类型：
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作者：
Mathers T
The banana aphid,Pentalonia nigronervosaCoquerel (Hemiptera: Aphididae), is a major pest of cultivated bananas (Musaspp., order Zingiberales), primarily due to its role as a vector ofBanana bunchy top virus(BBTV), the most severe viral disease of banana worldwide. Here, we generated a highly complete genome assembly ofP. nigronervosa using a single PCR-free Illumina sequencing library. Using the same sequence data, we also generated complete genome assemblies of theP. nigronervosa symbiotic bacteriaBuchnera aphidicolaandWolbachia. To improve our initial assembly ofP. nigronervosa we developed a k-mer based deduplication pipeline to remove genomic scaffolds derived from the assembly of haplotigs (allelic variants assembled as separate scaffolds). To demonstrate the usefulness of this pipeline, we applied it to the recently generated assembly of the aphidMyzus cerasi, reducing the duplication of conserved BUSCO genes by 25%. Phylogenomic analysis ofP. nigronervosa, our improvedM. cerasiassembly, and seven previously published aphid genomes, spanning three aphid tribes and two subfamilies, reveals thatP. nigronervosa falls within the tribe Macrosiphini, but is an outgroup to other Macrosiphini sequenced so far. As such, the genomic resources reported here will be useful for understanding both the evolution of Macrosphini and for the study ofP. nigronervosa. Furthermore, our approach using low cost, high-quality, Illumina short-reads to generate complete genome assemblies of understudied aphid species will help to fill in genomic black spots in the diverse aphid tree of life.
