Chitinase expression in parotid glands of non-obese diabetic mice
Chitinase expression in parotid glands of non-obese diabetic mice
复制标题
DOI：
10.1111/j.1601-0825.2012.01904.x
复制
发表时间：
2012-07-01
期刊：
影响因子：
3.8
通讯作者：
Mataga, I.
中科院分区：
文献类型：
--
作者：
Fukushima, T.;Nashida, T.;Mataga, I.
Oral Diseases（2012）18，506512目的：本研究是使用口干症小鼠模型来鉴定唾液中口干症的蛋白质生物标志物的基础研究。我们确定了非肥胖糖尿病小鼠和对照小鼠腮腺中表达不同的基因;随后，我们研究了腮腺和唾液中这些基因编码的蛋白质的表达。材料与方法：采用基因芯片和实时荧光定量PCR技术检测NOD/ShiJcl和C57 BL/6 JJcl（对照）雌性小鼠腮腺或腮腺腺泡细胞基因表达的差异。随后，使用免疫印迹和免疫组织化学评估蛋白质表达。类似地，使用酶谱法评估唾液中的酶活性。结果如下：基于基因表达分析，Chia在糖尿病小鼠中的表达高于非糖尿病小鼠和对照小鼠;同样，Chia编码的蛋白质几丁质酶在糖尿病小鼠中的表达也更高。NOD/ShiJcl小鼠的唾液比对照小鼠的唾液具有更多几丁质酶。结论：糖尿病小鼠腮腺腺泡细胞中几丁质酶的表达明显高于非糖尿病小鼠和正常对照小鼠。几丁质酶表达和酶活性的增加可能是小鼠自身免疫性糖尿病的特征;然而，需要进一步的研究来评估其作为人类口干症生物标志物的用途。
Oral Diseases (2012) 18, 506512 Objective: This investigation was a basal study that used a mouse model of xerostomia to identify protein biomarkers of xerostomia in saliva. We identified genes expressed differently in parotid glands from non-obese diabetic mice with diabetes and those from control mice; subsequently, we investigated expression of the proteins encoded by these genes in parotid glands and saliva. Materials and Methods: DNA microarray and real-time PCR analyses were performed to detect differences between NOD/ShiJcl and C57BL/6JJcl (control) female mice in gene expression from parotid glands or parotid acinar cells. Subsequently, protein expression was assessed using immunoblotting and immunohistochemistry. Similarly, enzyme activity in saliva was assessed using zymography. Results: Based on gene expression analyses, Chia expression was higher in diabetic mice than non-diabetic mice and control mice; similarly, expression of chitinase, the protein encoded by Chia, was higher in diabetic mice. Saliva from NOD/ShiJcl mice had more chitinase than saliva from control mice. Conclusions: Chitinase was highly expressed in parotid acinar cells from diabetic mice compared with non-diabetic and control mice. Increased chitinase expression and enzyme activity may characterize the autoimmune diabetes in mice; however, further investigation is required to assess its use as a biomarker of xerostomia in humans.