Microbial sensors for small molecules: Development of a mevalonate biosensor
Microbial sensors for small molecules: Development of a mevalonate biosensor
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DOI：
10.1016/j.ymben.2006.08.002
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发表时间：
2007-01-01
影响因子：
8.4
通讯作者：
Keasling, Jay D.
中科院分区：
文献类型：
--
作者：
Pfleger, Brian F.;Pitera, Douglas J.;Keasling, Jay D.
我们描述了一种新的生物传感器菌株，用于检测和定量小分子甲氧戊酸。该生物传感器菌株是一种表达绿色荧光蛋白并通过改变生长速度来报告生长介质中甲羟戊酸浓度的大肠杆菌甲氧戊酸辅助菌。为了利用该生物传感器菌株在细胞外环境中进行高通量筛选(HTS)和定量测定甲羟戊酸，建立了甲羟戊酸对生长速率的依赖模型。一般来说，这种方法应该适用于任何可以开发出营养缺陷体的小分子的量化，并将对没有现成的筛选或选择的进化代谢途径的HTS有用。(C)2006 Elsevier Inc.保留所有权利。
We describe a novel biosensor strain for detection and quantification of a small molecule, mevalonate. The biosensor strain is an Escherichia coli mevalonate auxotroph that expresses the green fluorescent protein and reports on the mevalonate concentration in the growth medium through a change in growth rate. A model describing the growth rate dependence on mevalonate was developed in order to use the biosensor strain for high-throughput screening (HTS) and quantitative measurement of mevalonate in the extracellular environment. In general, this method should be applicable to the quantification of any small molecule for which an auxotroph can be developed and will be useful for HTS of evolved metabolic pathways for which there is no readily available screen or selection. (C) 2006 Elsevier Inc. All rights reserved.