Molecular dissection of the genetic relationships of source, sink and transport tissue with yield traits in rice
Molecular dissection of the genetic relationships of source, sink and transport tissue with yield traits in rice
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DOI：
10.1007/s00122-002-1113-z
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发表时间：
2003-02-01
影响因子：
5.4
通讯作者：
Zhang, Q
中科院分区：
文献类型：
--
作者：
Cui, KH;Peng, SB;Zhang, Q
同化物的源、汇和转运能力在籽粒产量形成过程中起着重要作用。本研究通过对代表水稻源库运输组织性状的QTL及其不同比例的分析，研究了这些性状的遗传基础及其与产量性状的关系。遗传材料是由在中国种植最广泛的杂交水稻的亲本珍珊97和明恢63杂交而成的重组自交系群体。利用221个分子标记座位构建了总长1,796 cM的连锁图谱，共检测到81个QTL，其中3个叶面积为源，每穗总颖花数为库，茎中大维管束数为运输组织，3个源库比，3个运输组织库比，1个运输组织库比和3个产量性状。单个QTL的解释变异量在1.12%~24.14%之间。共检测到5个表现与环境互作效应的QTL，其贡献率在3.19%~9.15%之间。结果表明，紧密连锁或多效性是籽粒产量性状与源、库、运输组织及其不同比例相关的遗传基础。在检测到的25个源-库-运输组织性状QTL和43个不同比率的QTL中，分别有8个和22个QTL被定位到含有产量性状特别是粒重QTL的相似基因组区块。将产量性状的QTL与源、库和运输组织间的QTL定位在一起，可能为产量性状的生理表达提供遗传解释，同时也表明，源、库和运输组织之间比率的改善可能会导致产量潜力的提高。
Source, sink, and translocation capacity of assimilates play important roles during the formation of grain yield. The present study was conducted to characterize the genetic bases of traits representing source, sink and transport tissue, and their relationships with yield traits in rice, by analyzing QTLs for these traits and various ratios among them. The genetic materials were a recombinant inbred population derived from a cross between two indica cultivars Zhenshan 97 and Minghui 63, the parents of the most-widely grown hybrid rice in China. Using a linkage map that covers a total of 1,796 cM based on 221 molecular marker loci, a total of 81 QTLs were identified for the 15 traits studied (three leaf areas as the source, total spikelets per panicle as the sink, the number of large vascular bundles in the stem as transport tissue, three source to sink ratios, three transport tissue to source ratios, one transport tissue to sink ratio and three yield traits). The amount of variation explained by individual QTLs ranged from 1.12% to 24.14%. Five QTLs were identified to show interaction effects with the environment, which explained from 3.19% to 9.15% of the variation. The results showed that close linkage or pleiotropy is the genetic basis for the correlations of grain yield traits with source, sink, transport tissue and the various ratios among them. Of the 25 QTLs identified for source-sink-transport tissue trait, and 43 for various ratios, 8 and 22 QTLs, respectively, were mapped to the similar genomic blocks harboring QTLs for yield traits, especially for grain weight. Co-location of QTLs for yield traits with those for ratios among source, sink and transport tissue may provide a genetic explanation for the physiological expression of yield traits, and also suggest that improvement in ratios among source, sink and transport tissue may result in improvement in yield potential.