BMPRII is a direct target of miR-21
BMPRII is a direct target of miR-21
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DOI：
10.1093/abbs/gmp049
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发表时间：
2009-07-01
影响因子：
3.7
通讯作者：
Jin, Youxin
中科院分区：
文献类型：
--
作者：
Qin, Wenming;Zhao, Botao;Jin, Youxin
MicroRNAs(MiRNAs)是一类小的非编码RNA，在转录后阶段调节同源基因的表达。虽然已知有几个miRNAs参与了多种生物学过程，包括发育计时、模式形成、胚胎发生、分化和器官发生、生长控制和细胞凋亡，但许多靶基因和大多数miRNAs的功能仍不清楚。由于miRNAs与其靶标在动物细胞中只有部分互补性，因此很难确定特定miRNA的特定靶标基因并阐明其功能。在本研究中，我们证实了骨形态发生蛋白II受体(BMPRII)是miR-21的直接靶点，并表明BMPRII的蛋白水平与PC3和LNCaP细胞中miR-21的含量呈负相关。这些发现提示miR-21可能通过调节BMPRII的表达而在调节前列腺癌细胞的恶性和转移能力以及干细胞的自我更新方面发挥潜在的作用。
MicroRNAs (miRNAs) are a type of small non-coding RNAs that regulate cognate mRNA expressions at the post-transcriptional stage. Although several miRNAs are known to be involved in various biological processes, including developmental timing, patterning, embryogenesis, differentiation and organogenesis, growth control, and apoptosis, many target genes and the functions of most miRNAs are still unclear. Since there is only a partial complementarity between miRNAs and their targets in animal cells, it is difficult to identify the specific target genes for a given miRNA and elucidate its function. In this study, we confirmed that bone morphogenetic protein receptor II (BMPRII) is a direct target of miR-21, and also showed that the protein level of BMPRII correlates inversely with the amount of miR-21 in PC3 and Lncap cells. These findings suggest that miR-21 may have a potential role in regulating the malignancy and metastatic abilities of prostate cancer cells and in self-renewal of stem cells by regulating the expression of BMPRII.