Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases
Cloning and characterization of DYRK1B, a novel member of the DYRK family of protein kinases
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DOI：
10.1006/bbrc.1998.9967
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发表时间：
1999-01-19
影响因子：
3.1
通讯作者：
Becker, W
中科院分区：
文献类型：
--
作者：
Leder, S;Weber, Y;Becker, W
人类21号染色体上的DYRK 1A基因编码一种蛋白激酶，推测其参与唐氏综合征精神发育迟滞的发病机制。在这里，我们描述了一个高度相似的同源物，DYRK 1B，这是相反，DYRK 1A，主要在肌肉和睾丸中表达。利用放射杂交技术将DYRK 1B基因定位于19号染色体19 q12 -13.11。DYRK 1A和DYRK 1B的氨基酸序列在N-末端和催化结构域中有84%相同，但在C-末端区域没有显示出延伸的序列相似性。DYRK 1B包含蛋白激酶DYRK家族特征性的所有基序。此外，该序列包含二分核定位基序。转染后的COS-7细胞核中主要可见DYRK 1 B的绿色荧光蛋白（GFP）融合蛋白。这些数据表明，DYRK 1B是肌肉和睾丸特异性的DYRK 1A亚型，并参与核功能的调节。(C)北京：科学出版社.
The DYRK1A gene on human chromosome 21 encodes a protein kinase presumed to be involved in the pathogenesis of mental retardation in Down's syndrome. Here we describe a highly similar homolog, DYRK1B, which is, in contrast to DYRK1A, predominately expressed in muscle and testis. The human DYRK1B gene was mapped to chromosome 19 (19q12-13.11) by radiation hybrid analysis. The amino acid sequences of DYRK1A and DYRK1B are 84% identical in the N-terminus and the catalytic domain but show no extended sequence similarity in the C-terminal region. DYRK1B contains all motifs characteristic for the DYRK family of protein kinases, In addition, the sequence comprises a bipartite nuclear localization motif. A green fluorescent protein (GFP) fusion protein of DYRK1B was found mainly in the nucleus of transfected COS-7 cells. These data suggest that DYRK1B is a muscle- and testis-specific isoform of DYRK1A and is involved in the regulation of nuclear functions. (C) 1999 Academic Press.