Optofluidic DNA computation based on optically manipulated microdroplets
Optofluidic DNA computation based on optically manipulated microdroplets
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DOI：
10.1007/s10404-012-0934-6
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发表时间：
2012-07-01
影响因子：
2.8
通讯作者：
Tanida, Jun
中科院分区：
文献类型：
--
作者：
Nishimura, Takahiro;Ogura, Yusuke;Tanida, Jun
DNA计算是处理生物分子信息的一种很有前途的方法。虽然已经提出了几种可以评估生物分子输入的DNA逻辑电路，但它们在处理速度和实施中使用的分子量方面存在严重缺陷。在这里，我们提出了一种有效的方法来构建一个DNA计算系统的光流DNA计算。通过将DNA计算的反应空间限制在一个微滴内部，并利用外部光信号操纵一组微滴，提高了DNA计算的可用性和处理性能。应用光学操纵来运输液滴并通过液滴的强制合并来启动DNA计算。与传统的DNA计算方案相比，该方法具有操作灵活、同时多路求值和处理加速等优点。作为第一个演示的光流DNA计算，逻辑与或操作进行的光学操纵的微滴包含DNA逻辑门或输入分子。此外，由于减少了微滴的反应空间，在光流体DNA计算上确认了处理时间的显著减少。
DNA computing is a promising approach for dealing with biomolecular information. Although several DNA logic circuits which can evaluate biomolecular inputs have been proposed, they have serious drawbacks in the processing speed and the amount of molecules used in implementation. Here, we present optofluidic DNA computation as an effective method for constructing a DNA computing system. By confining the reaction space of DNA computation to the inside of a microdroplet and manipulating a group of droplets with external light signals, we improve usability of DNA computation as well as the processing performance. Optical manipulation is applied to transport the droplets and to initiate DNA computation by forced merging of the droplets. The proposed method has advantages over conventional DNA computation schemes in flexible operations, simultaneous multiplexed evaluation, and processing acceleration. As the first demonstration of optofluidic DNA computation, logical AND and OR operations are performed by optical manipulation of microdroplets which contain either DNA logic gates or input molecules. Also, considerable reduction in the processing time is confirmed on the optofluidic DNA computation owing to reduction of the reaction space to the microdroplet.