Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization
Nesprin 4 is an outer nuclear membrane protein that can induce kinesin-mediated cell polarization
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DOI：
10.1073/pnas.0808602106
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发表时间：
2009-02-17
影响因子：
11.1
通讯作者：
Burke, Brian
中科院分区：
文献类型：
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作者：
Roux, Kyle J.;Crisp, Melissa L.;Burke, Brian
核质偶联由外核膜（ONM）nesprin蛋白和内核膜Sun蛋白介导。跨越核周空间的相互作用产生连接细胞骨架和核组分的nesprin-Sun复合物。通过对nesprin 1-3中保守的C-末端序列的蛋白质的搜索，鉴定出了nesprin 4（Nesp 4），这是该家族的一个新成员。Nesp 4是一种驱动蛋白-1结合蛋白，其显示出对ONM的Sun依赖性定位。Nesp 4的表达与细胞组织的巨大变化有关，包括中心体和高尔基体相对于细胞核的重新定位。这些作用可以完全由Nesp 4的驱动蛋白结合功能来解释。这意味着Nesp 4可能有助于微管依赖的核定位。
Nucleocytoplasmic coupling is mediated by outer nuclear membrane (ONM) nesprin proteins and inner nuclear membrane Sun proteins. Interactions spanning the perinuclear space create nesprin-Sun complexes connecting the cytoskeleton to nuclear components. A search for proteins displaying a conserved C-terminal sequence present in nesprins 1-3 identified nesprin 4 (Nesp4), a new member of this family. Nesp4 is a kinesin-1-binding protein that displays Sun-dependent localization to the ONM. Expression of Nesp4 is associated with dramatic changes in cellular organization involving relocation of the centrosome and Golgi apparatus relative to the nucleus. These effects can be accounted for entirely by Nesp4's kinesin-binding function. The implication is that Nesp4 may contribute to microtubule-dependent nuclear positioning.