CONVERSION OF ESTERIFIED FURA-2 AND INDO-1 TO CA-2+-SENSITIVE FORMS BY MITOCHONDRIA
CONVERSION OF ESTERIFIED FURA-2 AND INDO-1 TO CA-2+-SENSITIVE FORMS BY MITOCHONDRIA
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DOI：
10.1152/ajpcell.1988.255.3.c304
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发表时间：
1988-09-01
影响因子：
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通讯作者：
GUNTER, KK
中科院分区：
文献类型：
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作者：
GUNTER, TE;RESTREPO, D;GUNTER, KK
大鼠肝线粒体显示将乙酰氧基甲基酯形式的fura-2和indo-1转化为Ca 2+依赖性的fura-2乙酰氧基甲基酯转化产物显示为fura-2的五羧酸形式。这些指示剂的五羧酸形式的荧光的离子强度和pH依赖性的系统研究显示，在Ca 2+摄取后线粒体基质内被认为获得的范围内的微小变化。线粒体内游离Ca 2+水平的研究之前和之后的Ca 2+螯合线粒体，和一个粗略的估计是由线粒体的贡献的Ca 2+依赖的fura-2荧光的肝细胞悬浮液。
Rat liver mitochondria are shown to convert the acetoxymethyl ester forms of fura-2 and indo-1 into Ca2+-dependent conversion product of fura-2 acetoxymethyl ester is shown to that of the pentacarboxylic acid form of fura-2. A systematic investigation of the ionic strength and pH dependences of the fluorescence of the pentacarboxylic acid forms of these indicators shows small changes within the ranges thought to obtain within the mitochondrial matrix after Ca2+ uptake. Intramitochondrial free Ca2+ levels are studied both before and after Ca2+ sequestration by mitochondria, and a rough estimate is made of the mitochondrial contribution of the Ca2+-dependent fura-2 fluorescence of a hepatocyte suspension.