Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
Introducing mothur: Open-Source, Platform-Independent, Community-Supported Software for Describing and Comparing Microbial Communities
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DOI：
10.1128/aem.01541-09
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发表时间：
2009-12-01
影响因子：
4.4
通讯作者：
Weber, Carolyn F.
中科院分区：
文献类型：
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作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
Mothur的目标是成为一个全面的软件包，允许用户使用单一软件来分析社区序列数据。它建立在以前的工具之上，为分析测序数据提供了一个灵活而强大的软件包。作为一个案例研究，我们使用Mothur对序列进行修剪、筛选和比对；计算距离；将序列分配给可操作的分类单位；并描述了之前通过16S rRNA基因片段焦磷酸测序表征的8个海洋样本的α和β多样性。使用膝上型计算机在不到2小时内完成了对超过222,000个序列的分析。
mothur aims to be a comprehensive software package that allows users to use a single piece of software to analyze community sequence data. It builds upon previous tools to provide a flexible and powerful software package for analyzing sequencing data. As a case study, we used mothur to trim, screen, and align sequences; calculate distances; assign sequences to operational taxonomic units; and describe the alpha and beta diversity of eight marine samples previously characterized by pyrosequencing of 16S rRNA gene fragments. This analysis of more than 222,000 sequences was completed in less than 2 h with a laptop computer.