Kinetic Study on Solute Permeation at the Interface of Molecular Aggregates by Partial Filling Capillary Electrophoresis
Kinetic Study on Solute Permeation at the Interface of Molecular Aggregates by Partial Filling Capillary Electrophoresis
复制标题
部分填充毛细管电泳研究溶质在分子聚集体界面渗透的动力学
DOI：
10.1002/elps.202100252
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发表时间：
2021
期刊：
影响因子：
2.9
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Miyabe;K.; Nakajima;M.
采用部分填充毛细管电泳矩分析法研究了溶质在球形分子聚集体界面的渗透动力学。根据溶质和分子聚集体的迁移速度将毛细管电泳体系分为5类，建立了部分填充毛细管电泳的矩方程。将该方法应用于电中性溶质在SDS胶束中的溶解研究。通过改变SDS浓度和SDS胶束区对毛细管的填充比（SDS胶束填充比），采用部分填充毛细管电泳法测定洗脱峰。利用矩方程，由洗脱峰的一阶绝对矩和二阶中心矩确定了SDS胶束的分配平衡常数（Kp）和界面溶质渗透速率常数（kinandkout）。它们的值是相当的，无论是在何种情况下，几乎与以前通过完全填充CE测量的值相同。用kin-out和kout的变化解释了Kp与溶质疏水性的正相关性。结果表明，采用部分填充毛细管电泳的矩分析方法是研究溶质在球形分子聚集体界面渗透动力学的有效方法。
Moment analysis method using partial filling CE was developed for the kinetic study on solute permeation at the interface of spherical molecular aggregates. Moment equations for partial filling CE were developed by classifying CE systems into five categories according to the migration velocities of solute and molecular aggregate. The method was applied to the study on the dissolution of electrically neutral solutes into SDS micelles. Elution peaks were measured by partial filling CE while changing the concentration of SDS and the filling ratio of SDS micellar zone to the capillary (ϕM). Partition equilibrium constants (Kp) and rate constants of interfacial solute permeation of SDS micelles (kinandkout) were determined from the first absolute and second central moments of the elution peaks by using the moment equations. Their values were comparable irrespective ofϕMand were almost the same as those previously measured by complete filling CE. The positive correlation ofKpwith the hydrophobicity of the solutes was explained in terms of the change inkinandkout. It was demonstrated that the moment analysis method using partial filling CE is effective for studying solute permeation kinetics at the interface of spherical molecular aggregates.