Ena/VASP: towards resolving a pointed controversy at the barbed end
Ena/VASP: towards resolving a pointed controversy at the barbed end
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DOI：
10.1242/jcs.038125
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发表时间：
2009-06-15
影响因子：
4
通讯作者：
Gertler, Frank B.
中科院分区：
文献类型：
--
作者：
Bear, James E.;Gertler, Frank B.
Ena/VASP蛋白是肌动蛋白动力学的保守调节剂，其在若干生理过程中具有重要作用，例如形态发生、轴突引导、内皮屏障功能以及癌细胞侵袭和转移。虽然有相当多的证据指向Ena/VASP功能的抗加帽机制，但仍存在一些争议。在这里，我们评估了支持和反对反加帽假说的证据，包括最近一些结构和生化研究的结果，这些研究为这个问题提供了新的线索。此外，我们描述了几种替代机制，Ena/VASP蛋白可能利用调节肌动蛋白在体内的动力学，包括抑制分支，捆绑和profilin-actin招聘。
Ena/VASP proteins are conserved regulators of actin dynamics that have important roles in several physiological processes such as morphogenesis, axon guidance, endothelial barrier function, and cancer cell invasion and metastasis. Although considerable evidence points towards an anti-capping mechanism for Ena/VASP function, some controversy remains. Here, we evaluate the evidence for and against the anti-capping hypothesis, including results from some recent structural and biochemical studies that shed new light on this issue. In addition, we describe several alternate mechanisms that Ena/VASP proteins may utilize to regulate actin dynamics in vivo, including inhibition of branching, bundling and profilin-actin recruitment.