Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419
Complete genome sequence of the Medicago microsymbiont Ensifer (Sinorhizobium) medicae strain WSM419
复制标题
DOI：
10.4056/sigs.43526
复制
发表时间：
2010-01-01
影响因子：
--
通讯作者：
Howieson, John
中科院分区：
文献类型：
--
作者：
Reeve, Wayne;Chain, Patrick;Howieson, John
Ensifer (中华根瘤菌) medicae 是多种一年生苜蓿 (medicago) 物种的有效固氮微共生体。菌株 WSM419 是一种需氧、能动、不形成孢子的革兰氏阴性杆状菌株，是从 1981 年在意大利撒丁岛收集的骨螺根瘤中分离出来的。由于其固氮、腐生能力和耐酸特性，WSM419 在 1985 年至 1993 年间在澳大利亚商业化生产，作为年度医务人员的接种剂。在这里，我们描述了该生物体的基本特征，以及完整的基因组序列和注释。这是适应酸性土壤的一年生药用植物群的微共生体的完整基因组序列的第一份报告。我们发现其基因组大小为 6,817,576 bp，编码 6,518 个蛋白质编码基因和 81 个仅 RNA 编码基因。该基因组包含一条大小为 3,781,904 bp 的染色体和 3 个大小为 1,570,951 bp、1,245,408 bp 和 219,313 bp 的质粒。最小的质粒是这种医疗微共生体的独特特征。
Ensifer (Sinorhizobium) medicae is an effective nitrogen fixing microsymbiont of a diverse range of annual Medicago (medic) species. Strain WSM419 is an aerobic, motile, non-spore forming, Gram-negative rod isolated from a M. murex root nodule collected in Sardinia, Italy in 1981. WSM419 was manufactured commercially in Australia as an inoculant for annual medics during 1985 to 1993 due to its nitrogen fixation, saprophytic competence and acid tolerance properties. Here we describe the basic features of this organism, together with the complete genome sequence, and annotation. This is the first report of a complete genome sequence for a microsymbiont of the group of annual medic species adapted to acid soils. We reveal that its genome size is 6,817,576 bp encoding 6,518 protein-coding genes and 81 RNA only encoding genes. The genome contains a chromosome of size 3,781,904 bp and 3 plasmids of size 1,570,951 bp, 1,245,408 bp and 219,313 bp. The smallest plasmid is a feature unique to this medic microsymbiont.