Effect of Setting Atmosphere on Apatite Cement Resorption: An In Vitro and In Vivo Study
Effect of Setting Atmosphere on Apatite Cement Resorption: An In Vitro and In Vivo Study
复制标题
环境气氛对磷灰石骨水泥吸收的影响：体外和体内研究
DOI：
10.1016/j.jmbbm.2018.08.021
复制
发表时间：
2018
影响因子：
3.9
通讯作者：
Kunio Ishikawa
中科院分区：
文献类型：
--
作者：
Arief Cahyanto;Kanji Tsuru;Kunio Ishikawa
本研究旨在探讨环境气氛对人工骨替代磷灰石骨水泥的影响。材料与方法将磷酸四钙和无水磷酸氢钙等摩尔比的磷灰石水泥与蒸馏水混合，在0%、5%和100%二氧化碳气氛下，在37 °C和100%相对湿度下固化。用X射线衍射仪和傅里叶变换红外光谱仪证实了碳酸盐磷灰石的形成。对12只10周龄健康雄性Wistar大鼠(S)在1个月和6个月时进行显微CT和组织学检查。结果在100%CO2和5%CO2条件下，磷灰石骨水泥形成B型碳酸盐磷灰石。与5%CO2相比，在100%CO2条件下有较多的碳酸盐磷灰石生成，而在0%CO2条件下没有生成碳酸盐磷灰石。有趣的是，当磷灰石水泥在0%CO2下固化时，未反应的磷酸四钙是显著的，表明形成了缺钙的羟基磷灰石。在磷灰石骨水泥和烧结型羟基磷灰石的条件下重建大鼠胫骨缺损区时，6个月后可见磷灰石骨水泥被替换，而烧结型羟基磷灰石未见替换。骨替代磷灰石骨水泥的量较大，分别为100%CO2和5%CO2，其次为0%CO2。结论实验结果表明，固化气氛对固化磷灰石骨水泥的骨替代起着重要作用。
ObjectivesThe aim of this present study was to investigate the effect of setting atmosphere on replacement of apatite cement with new bone bothin vitroandin vivo.Material and methodsApatite cement consisting of an equimolar mixture of tetracalcium phosphate and anhydrous dicalcium phosphate was mixed with distilled water and allowed to set at 37 °C and 100% relative humidity under 0%,5%, and 100% CO2atmospheres. X-Ray diffraction and Fourier Transform Infrared Spectroscopy were employed to confirm the carbonate apatite formation. Micro-CT and histological evaluation was made at 1 and 6 month(s) using twelve 10-week-old specific-pathogen-free male Wistar rats.ResultsB-type carbonate apatite was found when the apatite cement was set under 100% CO2and 5% CO2. More carbonate apatite was formed in the case of 100% CO2when compared with 5% CO2, and none was formed under 0% CO2. Interestingly, unreacted tetracalcium phosphate was significant when apatite cement was set under 0% CO2, indicating the formation of Ca-deficient hydroxyapatite. When a bone defect of rat tibia was reconstructed in these conditions of apatite cement and sintered hydroxyapatite, replacement of the apatite cement was confirmed 6 months after implantation, whereas no replacement was observed in the case of sintered hydroxyapatite. The amount of replacement of apatite cement with bone was greater, on the order of 100% CO2and 5% CO2, followed by 0% CO2.ConclusionThe results obtained in the present study demonstrated that setting atmosphere clearly plays an important role in the replacement of set apatite cement with bone.