Dysregulation of miR-146a by periodontal pathogens: A risk for acute coronary syndrome
Dysregulation of miR-146a by periodontal pathogens: A risk for acute coronary syndrome
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DOI：
10.1002/jper.18-0466
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发表时间：
2019-07-01
影响因子：
4.3
通讯作者：
Gnanamani, A.
中科院分区：
文献类型：
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作者：
Gita, J. Bagavad;George, Ann, V;Gnanamani, A.
背景牙周炎是一种由多种微生物引起的慢性炎症性疾病，可导致牙齿支持结构的丧失。菌血症、内毒素血症和全身性低度炎症将牙周炎与全身性疾病如糖尿病和冠状动脉疾病相关联。在聚合酶链反应中，通过使用特异性寡核苷酸引物扩增遗传物质，从动脉粥样硬化斑块中检测出牙周病病原体。虽然牙周炎和心血管疾病之间的关联已被系统评价和荟萃分析所证实，但其病理生理学尚不清楚。MicroRNA目前参与了许多细胞过程的调节，包括炎症，并可能在我们理解这种疾病相关性方面发挥重要作用。在这项病例对照研究中，我们探讨了炎性microRNA，miR-146 a，在急性冠状动脉综合征（ACS）的受试者和慢性牙周炎（CP）和其调节先天免疫宿主对牙周病原体的反应的作用。方法将ACS患者分为3组，每组66例，即ACS无CP组、ACS合并CP组和ACS单纯CP组。对龈下菌斑样本和血清样本进行定量聚合酶链反应（qPCR），以检测牙龈卟啉单胞菌（一种关键病原体），并评估循环miR-146 a和相关促炎细胞因子的水平。结果miR-146 a在第2组中的OR值为1.434，95%可信区间为1.013-2.030，P < 0.042，预测百分比为83.3%，在第1组中的预测百分比为76.0%。相关的细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α和IL-1 β也显示具有统计学显著性的上调（P < 0.05）。结论microRNA-146 a是牙周炎与急性冠状动脉综合征相关的关键分子。
Background Periodontitis is a polymicrobial, chronic inflammatory disease leading to loss of tooth-supporting structures. The bacteremia, endotoxemia, and systemic low-grade inflammation associate periodontitis with systemic illnesses such as diabetes mellitus and coronary artery disease. Periodontal pathogens have been detected from atheromatous plaque by amplification of the genetic material by using specific oligonucleotide primers in polymerase chain reaction. Though the association between periodontitis and cardiovascular diseases has been ascertained by systematic reviews and meta-analyses, its pathophysiology is not lucid. MicroRNAs are currently implicated in the regulation of many cellular processes including inflammation and may play a vital role in our understanding of this disease association. In this case-control study, we explored the role of the inflammatory microRNA, miR-146a, in acute coronary syndrome (ACS) subjects with and without chronic periodontitis (CP) and its regulation of the innate immune host response to periodontal pathogens. Methods Three groups each comprising 66 patients each, namely group 1 (ACS patients without CP), group 2 (ACS patients with CP) and group 3 (CP only) formed the study population. Subgingival plaque samples and serum samples were subjected to quantitative Polymerase Chain Reaction (qPCR) for detection of Porphyromonas gingivalis, a keystone pathogen and to assess the levels of circulating miR-146a and associated proinflammatory cytokines. Results miR-146a associated significantly in group 2 subjects with an odds ratio 1.434, 95% confidence interval 1.013-2.030, P < 0.042, and a predictive percentage of 83.3% and group 1 with a predictive percentage of 76.0.% The associated cytokines interleukin-6 (IL-6), tumor necrosis factor-alpha, and IL-1 beta also showed an upregulation with statistical significance (P < 0.05). Conclusion microRNA-146a is a key molecule associating periodontitis with acute coronary syndrome.