Co-utilization of glycerol and lignocellulosic hydrolysates enhances anaerobic 1,3-propanediol production by Clostridium diolis.
Co-utilization of glycerol and lignocellulosic hydrolysates enhances anaerobic 1,3-propanediol production by Clostridium diolis.
复制标题
甘油和木质纤维素水解产物的共同利用增强了二醇梭菌的厌氧 1,3-丙二醇生产。
DOI：
10.1038/srep19044
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Xu Ping
中科院分区：
文献类型：
--
作者：
Xin Bo;Wang Yu;Tao Fei;Li Lixiang;Ma Cuiqing;Xu Ping
使用木质纤维素水解产物作为共底物的厌氧发酵是一种经济上有吸引力的方法，可通过提高甘油的转化率来提高 1,3-丙二醇 (1,3-PD) 的产量。木质纤维素水解产物含有混合糖，主要是葡萄糖、木糖和阿拉伯糖。因此，在 Diolisstrain DSM 15410 生产 1,3-PD 时，分别使用这三种糖作为甘油的共底物，导致 1,3-PD 产量增加 18%–28%。混合糖和甘油的共发酵获得了更高的细胞内 NADH/NAD+ 比率，并且相对于单独甘油发酵，1,3-PD 产量增加了 22%。此后，将两种木质纤维素水解产物——玉米秸秆水解产物和玉米芯糖蜜——分别与甘油共发酵。从甘油中得到的1,3-PD的最大产率达到0.85mol/mol。还进行了补料分批共发酵，提高了 1,3-PD 产量（从 0.62 mol/mol 提高到 0.82 mol/mol）。这些结果表明，共发酵策略是从甘油生产 1,3-PD 的一种有效且经济的方法。
Anaerobic fermentation using lignocellulosic hydrolysates as co-substrates is an economically attractive method to enhance 1,3-propanediol (1,3-PD) production by increasing the conversion yield from glycerol. Lignocellulosic hydrolysates contain the mixed sugars that are primarily glucose, xylose and arabinose. Therefore, these three individual sugars were used, separately, as co-substrates with glycerol, in 1,3-PD production by aClostridium diolisstrain DSM 15410, resulting in an 18%–28% increase in the 1,3-PD yield. Co-fermentation of the mixed sugars and glycerol obtained a higher intracellular NADH/NAD+ratio and increased the 1,3-PD yield by 22% relative to fermentation of glycerol alone. Thereafter, two kinds of lignocellulosic hydrolysates, corn stover hydrolysate and corncob molasses, were individually co-fermented with glycerol. The maximum 1,3-PD yield from glycerol reached 0.85 mol/mol. Fed-batch co-fermentation was also performed, improving the 1,3-PD yield (from 0.62 mol/mol to 0.82 mol/mol). These results demonstrate that the co-fermentation strategy is an efficient and economical way to produce 1,3-PD from glycerol.