SUPPRESSION OF ENDOGENOUS AVIDIN-BINDING ACTIVITY IN TISSUES AND ITS RELEVANCE TO BIOTIN-AVIDIN DETECTION SYSTEMS
SUPPRESSION OF ENDOGENOUS AVIDIN-BINDING ACTIVITY IN TISSUES AND ITS RELEVANCE TO BIOTIN-AVIDIN DETECTION SYSTEMS
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DOI：
10.1177/29.10.7028859
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
WARNKE, R
中科院分区：
文献类型：
--
作者：
WOOD, GS;WARNKE, R
随着生物素-亲和素系统在单细胞、细胞悬浮液和特别是组织切片方面的应用不断发展，需要一种阻断内源性亲和素结合活性的方法。本文描述了一种这样的方法，并讨论了其提出的机理。利用该方法，在选定的人和小鼠组织中检测和抑制内源性亲和素结合活性，从而便于在生物素-亲和素-辣根过氧化物酶检测系统中利用杂杂瘤单克隆抗体解释特异性免疫组织化学染色。
As biotin-avidin systems continue to be developed for applications involving single cells, cell suspensions and especially tissue sections, the need arises for a method of blocking endogenous avidin-binding activity. One such method is described and its proposed mechanism is discussed. Utilizing this method, endogenous avidin-binding activity was detected and suppressed in selected human and murine tissues, thus facilitating the interpretation of specific immunohistochemical staining utilizing hybridoma monoclonal antibodies in a biotin-avidin-horseradish peroxidase detection system.