Cotransfer of Parthenogenetic Embryos Improves the Pregnancy and Implantation of Nuclear Transfer Embryos in Mouse
Cotransfer of Parthenogenetic Embryos Improves the Pregnancy and Implantation of Nuclear Transfer Embryos in Mouse
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DOI：
10.1089/clo.2008.0003
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发表时间：
2008-12-01
期刊：
影响因子：
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通讯作者：
Andras Dinnyes
中科院分区：
文献类型：
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作者：
Meng, Qinggang;Wang, Minkang;Andras Dinnyes
大多数体细胞核移植（SCNT）克隆在移植后或移植后死亡。提高足月健康妊娠率是SCNT技术的经济性、可行性和动物福利状况的关键问题。本研究探讨了孤雌或受精胚胎共移植对SCNT小鼠胚胎妊娠和着床的影响。通过将卵丘细胞核转移到去核的B6 D2 F1小鼠卵母细胞中产生SCNT胚胎，而孤雌激活（PA）和受精胚胎分别来自ICR小鼠通过锶人工激活和体内受精。与PA或受精胚胎相比，SCNT胚胎的囊胚发育能力较差。将SCNT胚胎单独移植（SCNT）、与2 ~ 3个PA（SCNT + PA）或受精（SCNT + Fert）胚胎共移植到ICR受体的输卵管中。SCNT + PA组克隆的妊娠率和种植率均显著高于SCNT组（p < 0.05）。在所有情况下，来自SCNT、SCNT + PA或SCNT + Fert的克隆的胎盘重量显著高于受精对照的胎盘重量（p < 0.001）。组织病理学观察表明，与PA或受精胚胎共移植的SCNT胚胎所获得的克隆大多数存活至成年，并具有生育能力和健康状况。我们的研究结果表明，在小鼠中，PA胚胎的共移植改善了SCNT胚胎的妊娠和植入，而不会损害所产生的克隆的整体健康。
The majority of somatic cell nuclear transfer (SCNT) clones dies in the peri- or postimplantation period. Improvement of the full-term healthy pregnancy rates is a key issue for the economical, viability and animal welfare profile of SCNT technology. In this study the effects of cotransfer of parthenogenetic or fertilized embryos on the pregnancy and implantation of SCNT mouse embryos have been investigated. SCNT embryos were produced by transferring cumulus cell nuclei into enucleated B6D2F1 mouse oocytes, whereas parthenogenetically activated (PA) and fertilized embryos were derived from ICR mice by artificial activation with strontium and in vivo fertilization, respectively. SCNT embryos were inferior in their developmental capacity to blastocyst compared to either PA or fertilized embryos. SCNT embryos were transferred alone (SCNT), or cotransferred with two to three PA (SCNT + PA) or fertilized (SCNT + Fert) embryos into the oviducts of an ICR recipient. Both pregnancy and implantation rates originating from clones in the SCNT + PA group were significantly higher than those of SCNT group (p < 0.05). The weight of placentas of clones derived from SCNT, SCNT + PA, or SCNT + Fert was in all cases significantly higher than that of fertilized controls (p < 0.001). Most of the clones derived from SCNT embryos cotransferred with PA or fertilized embryos Survived to adulthood and were fertile and healthy according to histopathological observations. Our results demonstrate in mouse that cotransfer of PA embryos improves the pregnancy and implantation of SCNT embryos without compromising the overall health of the resulting clones.