Adsorption Myoglobin over Mesoporous Silica Molecular Sieves: Pore Size Effect and Pore-Filling Model
Adsorption Myoglobin over Mesoporous Silica Molecular Sieves: Pore Size Effect and Pore-Filling Model
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DOI：
10.1016/j.msec.2006.05.012
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发表时间：
2007-03
期刊：
影响因子：
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通讯作者：
Masahiko Miyahara;A. Vinu;K. Ariga
中科院分区：
文献类型：
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作者：
Masahiko Miyahara;A. Vinu;K. Ariga
本文研究了不同孔径的介孔材料（SBA-15和MCM-41）对缓冲溶液中肌红蛋白的吸附。肌红蛋白的Langmuir型吸附发生在介孔通道的内表面上的单层覆盖。通过X射线衍射、氮气吸附/脱附和FT-IR光谱对吸附剂进行了表征。吸附的肌红蛋白的量显著地取决于介孔二氧化硅吸附剂的比孔体积和/或孔径。使用孔隙填充模型分析这些实验结果，表明肌红蛋白分子在SBA-15孔隙中填充良好。吸附前后的FT-IR光谱证实了吸附的肌红蛋白的结构稳定性。
The present work describes the adsorption of myoglobin over mesoporous materials with different pore diameters (SBA-15 and MCM-41) from buffered solutions. The Langmuir-type adsorption of myoglobin occurs with monolayer coverage on the inner surface of the mesoporous channels. These adsorbents were thoroughly characterized by X-ray diffraction, nitrogen adsorption/desorption and FT-IR spectroscopy before and after the myoglobin adsorption. The amount of myoglobin adsorbed significantly depends on the specific pore volume and/or the pore diameter of the mesoporous silica adsorbents. These experimental results were analyzed using pore-filling models, suggesting that myoglobin molecules are well-packed in the SBA-15 pores. FT-IR spectra before and after the adsorption confirm the structural stability of the adsorbed myoglobin.