Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets
Specific and reversible DNA-directed self-assembly of oil-in-water emulsion droplets
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DOI：
10.1073/pnas.1214386109
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发表时间：
2012-12-11
影响因子：
11.1
通讯作者：
Hanczyc, Martin M.
中科院分区：
文献类型：
--
作者：
Hadorn, Maik;Boenzli, Eva;Hanczyc, Martin M.
起源于微观构建块的特异性和可逆的DNA导向自组装的高阶结构为未来技术带来了巨大的希望。在这里，我们官能化的生物素化的软胶体水包油乳液液滴与生物素化的单链DNA寡核苷酸使用链霉亲和素作为中间接头。我们表明，这个模块化的连接系统的组件是稳定的，并诱导序列特异性聚集的二元混合物的乳液液滴。三个长度尺度，从而涉及：纳米级DNA碱基配对连接微观积木导致肉眼可见的宏观聚集体。聚集过程是可逆的，通过改变温度和电解质浓度，并通过添加竞争性寡核苷酸。通过随后的乳液液滴的再官能化来重置和重复使用该系统。因此，DNA指导的水包油乳液液滴的自组装为可编程和可回收的软材料提供了坚实的基础，这些软材料根据需要进行结构重排，并且应用范围从信息技术到医学。
Higher-order structures that originate from the specific and reversible DNA-directed self-assembly of microscopic building blocks hold great promise for future technologies. Here, we functionalized biotinylated soft colloid oil-in-water emulsion droplets with biotinylated single-stranded DNA oligonucleotides using streptavidin as an intermediary linker. We show the components of this modular linking system to be stable and to induce sequence-specific aggregation of binary mixtures of emulsion droplets. Three length scales were thereby involved: nanoscale DNA base pairing linking microscopic building blocks resulted in macroscopic aggregates visible to the naked eye. The aggregation process was reversible by changing the temperature and electrolyte concentration and by the addition of competing oligonucleotides. The system was reset and reused by subsequent refunctionalization of the emulsion droplets. DNA-directed self-assembly of oil-in-water emulsion droplets, therefore, offers a solid basis for programmable and recyclable soft materials that undergo structural rearrangements on demand and that range in application from information technology to medicine.