Identification and characterization of SHORTENED UPPERMOST INTERNODE 1, a gene negatively regulating uppermost internode elongation in rice
Identification and characterization of SHORTENED UPPERMOST INTERNODE 1, a gene negatively regulating uppermost internode elongation in rice
复制标题
水稻最上部节间伸长负调控基因“SHORTENED UPPERMOST INTERNODE 1”的鉴定和表征
DOI：
10.1007/s11103-011-9825-6
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发表时间：
2011-11-01
影响因子：
5.1
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Zhu, Li;Hu, Jiang;Qian, Qian
在水稻中，伸长的节间来源于营养芽顶端分生组织（SAM），并且SAM从营养阶段向生殖阶段的过渡诱导节间伸长。在这项研究中，我们描述了两个缩短的最高节间突变体（sui 1 -1和sui 1 -2）。在苗期和分蘖期，sui 1植株在形态上与野生型植株相似。然而，在抽穗期，sui 1 -1突变体表现出缩短的最上部的节间和部分鞘穗，sui 1 -2突变体表现出极端缩短的最上部的节间和完全鞘穗。赤霉素处理导致每个节间伸长，但最上面的节间缩短的表型保持不变。显微分析表明，sui 1 -1最上部节间细胞长度减少。图位克隆显示SUI 1位于1号染色体上，编码一个假定的磷脂酰丝氨酸合酶（PSS）家族蛋白。在蛋白质数据库中的比对结果显示OsSUI 1含有InterPro结构域IPR 004277，该结构域在动物界和植物界都是保守的。野生型SUI 1基因的引入完全挽救了sui 1 -1和sui 1 -2的突变表型，证实了克隆基因的身份。与这些结果一致，SUI 1-RNAi转基因植物显示最上部节间的伸长减少。我们的研究结果表明，SUI 1在调节水稻最高节间长度通过减少纵向细胞长度的重要作用。
In rice, the elongated internodes are derived from the vegetative shoot apical meristem (SAM), and the transition of the SAM from the vegetative to the reproductive stage induces internode elongation. In this study, we characterize two shortened uppermost internode mutants (sui1-1 and sui1-2). During the seedling and tillering stages, sui1 plants are morphologically similar to wild-type plants. However, at the heading stage, the sui1-1 mutant exhibits a shortened uppermost internode and a partly sheathed panicle, and the sui1-2 mutant shows an extremely shortened uppermost internode and a fully sheathed panicle. Gibberellin treatment results in elongation of every internode, but the shortened uppermost internode phenotype remains unaltered. Microscopic analysis indicates that cell length of sui1-1 uppermost internode exhibits decreased. Map-based cloning revealed that SUI1 is located on Chromosome 1, and encodes a putative phosphatidyl serine synthase (PSS) family protein. Searches for matches in protein databases showed that OsSUI1 contains the InterPro domain IPR004277, which is conserved in both animal and plant kingdoms. Introduction of a wild-type SUI1 gene fully rescued the mutant phenotype of sui1-1 and sui1-2, confirming the identity of the cloned gene. Consistent with these results, the SUI1-RNAi transgenic plants displayed decreased elongation of the uppermost internode. Our results suggest that SUI1 plays an important role in regulating uppermost internode length by decreasing longitudinal cell length in rice.