Synaptotagmin III is a critical factor for the formation of the perinuclear endocytic recycling compartment and determination of secretory granules size
Synaptotagmin III is a critical factor for the formation of the perinuclear endocytic recycling compartment and determination of secretory granules size
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DOI：
10.1242/jcs.00186
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Sagi-Eisenberg, R
中科院分区：
文献类型：
--
作者：
Grimberg, E;Peng, Z;Sagi-Eisenberg, R
早期核内体和核周rab -11阳性室与内化受体的循环有关。在这项研究中，我们发现Syt蛋白家族的成员Syt III是形成和运送货物到核周内吞噬循环室（ERC）所必需的。研究表明，大鼠嗜碱性白血病（RBL-2H3）肥大细胞内源性表达Syt III，其中70%的Syt III蛋白与EEA1、膜联蛋白II和syntaxin 7等早期内体标志物共定位，其余的Syt III蛋白与分泌颗粒（SG）标志物共定位，如β -己糖氨酸酶、组胺和5 -羟色胺。为了研究Syt III的功能作用，我们用Syt III反义cDNA稳定转染RBL细胞，并监测细胞中转铁蛋白（Tfn）的内化途径。
Early endosomes and a perinuclear, Rab-11-positive compartment have been implicated in the recycling of internalized receptors. In this study, we show that synaptotagmin HI (Syt III), a member of the Syt family of proteins, is required for the formation and delivery of cargo to the perinuclear endocytic recycling compartment (ERC). We demonstrate that rat basophilic leukemia (RBL-2H3) mast cells endogenously express Syt III, and >70% of this protein colocalizes with early endosomal markers, such as EEA1, annexin II and syntaxin 7, and the remaining protein colocalizes with secretory granule (SG) markers such as (beta-hexosaminidase, histamine and serotonin. To study the functional role of Syt III, we stably transfected RBL cells with Syt III antisense cDNA and monitored the route of transferrin (Tfn) internalization in cells that displayed substantially reduced (