PROGESTERONE AND ADENOSINE-3',5'-MONOPHOSPHATE FORMATION BY ISOLATED HUMAN CORPORA-LUTEA OF DIFFERENT AGES - INFLUENCE OF HUMAN CHORIONIC-GONADOTROPIN AND PROSTAGLANDINS
PROGESTERONE AND ADENOSINE-3',5'-MONOPHOSPHATE FORMATION BY ISOLATED HUMAN CORPORA-LUTEA OF DIFFERENT AGES - INFLUENCE OF HUMAN CHORIONIC-GONADOTROPIN AND PROSTAGLANDINS
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DOI：
10.1210/jcem-55-1-102
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发表时间：
1982-01-01
影响因子：
5.8
通讯作者：
HAMBERGER, L
中科院分区：
文献类型：
--
作者：
DENNEFORS, BL;SJOGREN, A;HAMBERGER, L
在周期的黄体期的不同阶段，从34名接受小切口剖腹术的妇女中的每一个中，将黄体（CL）全部切除，去囊，切成碎片，并孵育短时间段（5-120分钟）。在不存在和存在hCG、前列腺素F2 α的情况下进行孵育。（PGF 2 α），和PGE 2，单独和组合。CL的年代测定是使用几个参数精心完成的。孵育后，测定cAMP的组织水平和孕酮（P）的培养基浓度。体外基础P产生量在黄体中期CL中最高。hCG刺激所有年龄的CL中cAMP的形成，在黄体中期的CL中产生最高水平的cAMP。PGE 2增加cAMP的形成和加强hCG的作用，在年轻的CL，但不是在CL的黄体中期。PGF 2 α，单独或与hCG组合，对年轻或年老CL中cAMP或P的形成没有影响，而在黄体中期的CL中，PGF 2 α对cAMP或P的形成没有影响。显著抵消hCG对cAMP和P生成的刺激作用。这些体外数据显示PGF 2 α.能够在人体内诱导功能性黄体溶解。
From each of 34 women undergoing minilaparotomy at various stages of the luteal phase of the cycle, the corpus luteum (CL) was excised in toto, decapsulated, cut into pieces, and incubated for short time periods (5-120 min). Incubations were conducted in the absence and presence of hCG, prostaglandin F2.alpha. (PGF2.alpha.), and PGE2, both alone and in combination. Dating of the CL was done meticulously using several parameters. After incubation, the tissue levels of cAMP and the media concentrations of progesterone (P) were determined. The basal P production in vitro was highest in CL of the midluteal phase. hCG stimulated cAMP formation in CL of all ages, with the highest levels of cAMP being produced in CL of the midluteal phase. PGE2 increased cAMP formation and potentiated the hCG effect in young CL, but not in CL of the midluteal phase. PGF2.alpha., alone or in combination with hCG, had no effect on cAMP or P formation in either young or old CL, while in CL of the midluteal phase, PGF2.alpha. significantly counteracted the stimulatory effect of hCG on both cAMP and P formation. These in vitro data show that PGF2.alpha. is capable of inducing functional luteolysis in humans.