PURIFICATION OF OLFACTORY NERVE ENSHEATHING CELLS FROM THE OLFACTORY-BULB
PURIFICATION OF OLFACTORY NERVE ENSHEATHING CELLS FROM THE OLFACTORY-BULB
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DOI：
10.1006/dbio.1993.1033
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发表时间：
1993-02-01
影响因子：
2.7
通讯作者：
NOBLE, M
中科院分区：
文献类型：
--
作者：
BARNETT, SC;HUTCHINS, AM;NOBLE, M
嗅球的嗅神经层的细胞用O 4抗体标记，O 4抗体还标记雪旺细胞、少突胶质细胞和许多少突胶质细胞2型星形胶质细胞（O-2A）祖细胞。从围产期大鼠的脊髓、海马、胼胝体和小脑中纯化O 4+，但不表达半乳糖苷（GC）的细胞总是产生具有O-2A祖细胞预期特征的细胞。相反，绝大多数O 4 + GC-细胞分离的嗅球表达的抗原表型和模式的分化在vitrowhich不符合任何先前描述的胶质细胞群体。由于O 4染色在嗅球中的定位表明这些细胞是嗅神经鞘细胞（ONECs），因此我们的研究结果表明ONECs代表了一种新的胶质细胞类型，现在可以作为纯化的群体进行分析。
Cells of the olfactory nerve layer of the olfactory bulb are labeled with the O4 antibody, which also labels Schwann cells, oligodendrocytes, and many oligodendrocyte-type-2 astrocyte (O-2A) progenitors. Purification of cells which were O4+, but did not express galactocerebroside (GC), from spinal cord, hippocampus, corpus callosum, and cerebellum of perinatal rats always yielded cells with the expected characteristics of O-2A progenitors. In contrast, the great majority of O4+GC-cells isolated from the olfactory bulb expressed an antigenic phenotype and pattern of differentiationin vitrowhich did not correspond with that of any previously described glial populations. As the localization of O4 staining in the olfactory bulb indicates that these cells are olfactory nerve ensheathing cells (ONECs), our results therefore suggest that ONECs represent a novel glial cell-type which can now be analyzed as a purified population.