Heat shock induces phosphorylation of histone H2AX in mammalian cells
Heat shock induces phosphorylation of histone H2AX in mammalian cells
复制标题
DOI：
10.1016/j.bbrc.2005.01.073
复制
发表时间：
2005-03-25
影响因子：
3.1
通讯作者：
Miura, M
中科院分区：
文献类型：
--
作者：
Kaneko, H;Igarashi, K;Miura, M
热休克诱导多种生物学事件，包括基因激活、细胞周期停滞和细胞凋亡。热休克最近已被证明是潜在的有用的，当结合辐射在癌症治疗中，可能是因为，在哺乳动物细胞中，热抑制电离辐射诱导的双链断裂（DSB）的修复。然而，目前尚不清楚热休克本身是否会诱导DSB。在这篇文章中，我们提出了热休克诱导组蛋白H2AX磷酸化形式的第一个证据，这被认为是在DSB位点附近的染色质中产生的。这些结果表明，热休克诱导哺乳动物细胞中的DSB，并可能提供直接的证据来解释以前的报告中的DSB相关事件发生后热休克处理。(C)2005年爱思唯尔公司All rights reserved.
Heat shock induces a variety of biological events including gene activation, cell cycle arrest, and apoptosis. Heat shock has recently been shown to be potentially useful when combined with radiation in cancer therapy, probably because, in mammalian cells, heat inhibits the repair of double-strand breaks (DSBs) induced by ionizing radiation. It remains unclear, however, whether heat shock by itself induces DSBs. In this Communication, we present the first evidence that heat shock induces the phosphorylated form of histone H2AX, which is thought to be generated at the chromatin proximal to DSB sites. These results suggest that heat shock induces DSBs in mammalian cells and may provide direct evidence to explain previous reports on DSB-related events occurring after heat shock treatment. (C) 2005 Elsevier Inc. All rights reserved.