Plant colonization by the vascular wilt fungus Fusarium oxysporum requires FOW1, a gene encoding a mitochondrial protein
Plant colonization by the vascular wilt fungus Fusarium oxysporum requires FOW1, a gene encoding a mitochondrial protein
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DOI：
10.1105/tpc.002576
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发表时间：
2002-08-01
期刊：
影响因子：
11.6
通讯作者：
Tsuge, T
中科院分区：
文献类型：
--
作者：
Inoue, I;Namiki, F;Tsuge, T
土传真菌尖孢镰刀菌（Fusarium oxysporum）通过直接穿透根并定殖维管组织而引起多种植物物种的维管枯萎。甜瓜枯萎病菌F. oxysporum F.以前通过限制性内切酶介导的DNA整合诱变分离出甜瓜黑胫病菌（sp.melonis）。B60的分子分析确定了受影响的基因，命名为FOW 1，它编码一种与酵母线粒体载体蛋白高度相似的蛋白质。虽然FOW1插入突变体和基因靶向突变体在培养中显示出正常的生长和分生孢子形成，但由于在植物组织中定殖能力的缺陷，它们显示出明显降低的毒力。使用表达禽绿荧光蛋白融合蛋白的菌株验证Fow1的线粒体输入。番茄枯萎病菌F. oxysporum F.番茄斑点病菌也表现出降低的毒力。这些数据有力地表明，FOW1编码一种线粒体载体蛋白，该蛋白是F.尖孢菌属
The soil-borne fungus Fusarium oxysporum causes vascular wilts of a wide variety of plant species by directly penetrating roots and colonizing the vascular tissue. The pathogenicity mutant B60 of the melon wilt pathogen F. oxysporum f. sp. melonis was isolated previously by restriction enzyme-mediated DNA integration mutagenesis. Molecular analysis of B60 identified the affected gene, designated FOW1, which encodes a protein with strong similarity to mitochondrial carrier proteins of yeast. Although the FOW1 insertional mutant and gene-targeted mutants showed normal growth and conidiation in culture, they showed markedly reduced virulence as a result of a defect in the ability to colonize the plant tissue. Mitochondrial import of Fow1 was verified using strains expressing the Fowl-green fluorescent protein fusion proteins. The FOW1-targeted mutants of the tomato wilt pathogen F. oxysporum f. sp. lycopersici also showed reduced virulence. These data strongly suggest that FOW1 encodes a mitochondrial carrier protein that is required specifically for colonization in the plant tissue by F. oxysporum.