Small intestinal growth caused by feeding red kidney bean phytohemagglutinin lectin to rats.
Small intestinal growth caused by feeding red kidney bean phytohemagglutinin lectin to rats.
复制标题
给大鼠喂食红芸豆植物血凝素凝集素引起的小肠生长。
DOI：
10.1016/0016-5085(93)90644-r
复制
发表时间：
1993
期刊：
影响因子：
29.4
通讯作者：
Abramowsky,C
中科院分区：
文献类型：
--
作者：
Banwell,JG;Howard,R;Kabir,I;Adrian,TE;Diamond,RH;Abramowsky,C
背景：植物凝集素大量存在于各种食物来源中。本研究的目的是，以确定他们是否刺激增长的intestin.Methods：无菌和传统的大鼠对喂养纯化的植物血凝素凝集素（PHA）或相当于酪蛋白在一个完全营养的饮食。将PHA滴入原位空肠和回肠袢中。结果：PHA在小肠（空肠大于回肠）产生营养反应（P< 0.001），持续暴露可维持营养反应（P< 0.001），恢复对照饮食后营养反应可逆转（P< 0.05）。常规动物肠道菌群植物群增加了PHA对肠重的促生长作用（P< 0.01）。PHA使无菌动物粪便蛋白质、脂肪和粘液糖蛋白水平升高（P< 0.001）。PHA可增加空肠粘膜隐窝深度和隐窝细胞分裂活性（P< 0.05），增加隐窝细胞DNA含量（P< 0.05）和[3H]胸腺嘧啶核苷摄取量（P< 0.01）。没有增加血浆或组织中的胃泌素，肠胰高血糖素，或肽YY的含量，观察PHA暴露，并没有增加肝脏，肾脏，或colon.Conclusions的器官重量：PHA刺激大鼠小肠的生长时，存在于饮食中或灌输在肠腔。
Background:Plant lectins are present in significant quantity in a variety of food sources. The aim of this study was to determine if they stimulated growth of the intestine.Methods:Germ-free and conventional rats were pair fed purified phytohemagglutinin lectin (PHA) or equivalent casein in a fully nutritious diet. PHA was instilled into in situ jejunal and ileal loops. Organ weight, length, DNA, protein content, morphometry, and [3H]thymidine uptake into jejunal crypt cells were measured.Results:A trophic response occurred in the small intestine (jejunum greater than ileum) because of PHA (P< 0.001), was sustained by continued exposure, and was reversible on reinstitution of the control diet (P< 0.05). The intestinal microbial flora in conventional animals that were fed PHA augmented the growth-stimulatory effects of PHA on intestinal weight (P< 0.01). PHA caused fecal protein, fat, and mucous glycoprotein levels (P< 0.001) to increase in germfree animals. PHA increased jejunal mucosal crypt depth and crypt mitotic activity (P< 0.05); DNA content (P< 0.05) and [3H]thymidine uptake (P< 0.01) into crypt cells was increased. No increase in plasma or tissue content of gastrin, enteroglucagon, or peptide YY was observed on PHA exposure, and there was no increase in organ weight of the liver, kidney, or colon.Conclusions:PHA stimulated growth of rat small intestine when present in the diet or instilled in the bowel lumen.