Characterization and Growing Development of Spodoptera exigua (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3h (HvAV-3h)
Characterization and Growing Development of Spodoptera exigua (Lepidoptera: Noctuidae) Larvae Infected by Heliothis virescens ascovirus 3h (HvAV-3h)
复制标题
感染夜蛾子囊病毒 3h (HvAV-3h) 的甜菜夜蛾（鳞翅目：夜蛾科）幼虫的特征和生长发育
DOI：
10.1093/jee/tow183
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发表时间：
2016-10-01
影响因子：
2.2
通讯作者：
Huang, Guo-Hua
中科院分区：
文献类型：
--
作者：
Hu, Jue;Wang, Xing;Huang, Guo-Hua
甜菜夜蛾（Spodoptera exigua，Hubner）是一种危害严重的世界性害虫，对许多农作物造成严重的经济损失。需要一种有效的、高毒力的病原微生物用作抗沙门氏菌的生物防治剂。甜菜夜蛾幼虫尤为重要。使用滴度为9.58 × 10（12）基因组拷贝/ml的含烟芽夜蛾囊病毒3 h（HvAV-3 h）的血淋巴进行PCR。每口施用甜菜幼虫，第一至第二龄每头幼虫1.06 x 10（10）个剂量，第三至第五龄每头幼虫9.58 x 10（9）个基因组拷贝。血体腔内注射的剂量为1.53 × 10（9）个基因组拷贝/3至5龄幼虫。注射后的死亡率，体重和食物摄入量的S。观察并记录甜菜幼虫。经口接种的1 ~ 5龄幼虫的校正死亡率分别为21.886 ± 0.98%、22.226 ± 4.00%、8.896 ± 4.01%、6.666 ± 3.33%和8.896 ± 2.94%。早龄期比晚龄期更易感染病毒。经注射接种的三龄、四龄和五龄幼虫的校正死亡率分别为96.586 ± 3.42%、98.836 ± 1.17%和97.786 ± 2.22%。与健康的幼虫群体相比，患病幼虫群体的存活时间显著延长。此外，摄食量大大减少，体重在第三和第四龄保持相当稳定。体重下降，在第五龄相应的食物摄入量减少。
Spodoptera exigua (Hubner) is a very serious worldwide pest capable of causing severe economic losses in numerous agricultural crops. The need for an effective, highly virulent, pathogenic microorganism for use as a biological control agent against S. exigua larvae is particularly important. Heliothis virescens ascovirus 3 h (HvAV-3h)-containing hemolymph with a titer of 9.58 x 10(12) genome copies per ml was used to inoculate S. exigua larvae per os with a 1.06 x 10(10) dosage per larva for the first-to second instar and 9.58 x 10(9) genome copies per larva for the third- to fifth instars. Intrahemocoelic injections were also used with a dosage of 1.53 x 10(9) genome copies per larva for third-to fifth instar. The postinjection mortality, body weight, and food intake of the S. exigua larvae were observed and recorded. The corrected mortality rates for the first-through fifth instar inoculated per os were 21.886 +/- 0.98, 22.226 +/- 4.00, 8.896 +/- 4.01, 6.666 +/- 3.33, and 8.896 +/- 2.94%, respectively. The early instars were significantly easier to infect with virus compared to the later instar. The corrected mortality of the third, fourth, and fifth instars inoculated by injection was 96.586 +/- 3.42, 98.836 +/- 1.17, and 97.786 +/- 2.22%, respectively. Compared to the healthy larval population, survival time of the diseased larval population was considerably extended. In addition, food intake was greatly reduced, and the body weight remained fairly constant in the third- and fourth instar. The body weight declined in the fifth instar corresponding to a reduction in food intake.