Integrating waveguide biosensor.
Integrating waveguide biosensor.
复制标题
集成波导生物传感器。
DOI：
10.1007/978-1-60327-567-5_22
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Karns,JeffreyS
中科院分区：
文献类型：
--
作者：
Li,Shuhong;Amstutz3rd,Platte;Tang,Cha-Mei;Hang,Jun;Zhu,Peixuan;Zhang,Yunqi;Shelton,DanielR;Karns,JeffreyS
The Integrating Waveguide Biosensor was developed for rapid and sensitive detection of bacterial cells, spores, and toxins. A sandwich format of immunoassay was employed usingSalmonellaas model. The analyte was immunocaptured on the inner surface of the waveguide and then detected by the antibody conjugated with fluorescent dye. The waveguide was illuminated by an excitation light at a 90° angle. The emitted light from fluorescent labels on the surface of the waveguide was efficiently collected and channeled to a detector at the end of the waveguide, while minimizing interference from the excitation light. Utilizing fluorescent dye Cy5, a 635-nm diode laser for excitation, and a photomultiplier tube detector, the Integrating Waveguide Sensor System was able to detect approximately ten captured cells ofSalmonella.
