Electronic supplementary material ( ESM )
Electronic supplementary material ( ESM )
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发表时间：
2020
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按照制造商的指示，使用 QIAmp 迷你试剂盒（QIAGEN，希尔登，德国）从 25 mg 速冻肝脏中提取 DNA，添加 PBS（80 μl）和缓冲液 ALT（180 μl）的体积略有变化以进行均质化。均质化 (Bead Ruptor Omni International) 并添加蛋白酶 K 后，在最终提取步骤之前将匀浆在室温下储存 11-14 天，这符合制造商 (Qiagen) 的说明，并且 DNA 甲基化在这种储存条件下是稳定的 [1, 2]。
DNA was extracted from 25 mg of snap frozen liver using the QIAmp mini kit (QIAGEN, Hilden, Germany) as indicated by the manufacturer with minor changes in volume of added PBS (80 μl) and buffer ALT (180 μl) for homogenization. After homogenization (Bead Ruptor Omni International) and addition of protease K, the homogenate was stored at room temperature for 11-14 days prior final extraction steps, which is in accordance with the instructions of the manufacturer (Qiagen) and DNA methylation was shown to be stable under such storage conditions [1, 2].