A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs.
A systematic analysis of the skeletal muscle miRNA transcriptome of chicken varieties with divergent skeletal muscle growth identifies novel miRNAs and differentially expressed miRNAs.
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对骨骼肌生长不同的鸡肉品种的骨骼肌 miRNA 转录组进行系统分析，鉴定出新的 miRNA 和差异表达的 miRNA
DOI：
10.1186/1471-2164-12-186
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发表时间：
2011-04-13
期刊：
影响因子：
4.4
通讯作者：
Zhu D
中科院分区：
文献类型：
--
作者：
Li T;Wu R;Zhang Y;Zhu D
研究背景功能研究表明microRNAs（miRNAs或miRs）在包括发育和疾病发病机制在内的广泛生物学过程中起着关键作用。为了研究miRNAs在鸡骨骼肌发育过程中发挥的功能作用，使用Solexa deep sequencing.ResultsSome miRNAs具有多种异构体和几种miRNAs* 存在于比其相应的miRNAs更高的水平上，对肉鸡和蛋鸡骨骼肌的miRNAs转录组进行了分析。使用计算方法鉴定了33种新的和189种已知的鸡miRNA。随后的miRNA转录组比较和实时PCR验证实验揭示了17个在肉鸡和蛋鸡之间差异表达的miRNA，并且这些miRNA的许多靶点与肌发生调控有关。利用整合的miRNA靶标预测和网络分析方法，构建了差异表达和肌肉相关的miRNA与其推定靶标的相互作用网络，并鉴定了可能通过靶向ACVR 2B基因促进肉鸡和蛋鸡肌肉分化生长的miRNA，结论本研究提供了第一个转录组谱-基于评估鸡骨骼肌发育过程中miRNA的功能。系统的预测有助于识别潜在的miRNAs及其靶点，这可能有助于肉鸡和蛋鸡的不同肌肉生长。此外，这些预测产生的信息可用于进一步研究包含miRNA及其靶点的相互作用网络参与肌肉发育的调节。
BackgroundFunctional studies have demonstrated that microRNAs (miRNAs or miRs) play critical roles in a wide spectrum of biological processes including development and disease pathogenesis. To investigate the functional roles that miRNAs play during chicken skeletal muscle development, the miRNA transcriptomes of skeletal muscles from broiler and layer chickens were profiled using Solexa deep sequencing.ResultsSome miRNAs have multiple isoforms and several miRNAs* are present at higher levels than their corresponding miRNAs. Thirty three novel and 189 known chicken miRNAs were identified using computational approaches. Subsequent miRNA transcriptome comparisons and real-time PCR validation experiments revealed 17 miRNAs that were differentially expressed between broilers and layers, and a number of targets of these miRNAs have been implicated in myogenesis regulation. Using integrative miRNA target-prediction and network-analysis approaches an interaction network of differentially expressed and muscle-related miRNAs and their putative targets was constructed, and miRNAs that could contribute to the divergent muscle growth of broiler and layer chickens by targeting theACVR2Bgene were identified, which can causes dramatic increases in muscle mass.ConclusionsThe present study provides the first transcriptome profiling-based evaluation of miRNA function during skeletal muscle development in chicken. Systematic predictions aided the identification of potential miRNAs and their targets, which could contribute to divergent muscle growth in broiler and layer chickens. Furthermore, these predictions generated information that can be utilized in further research investigating the involvement of interaction networks, containing miRNAs and their targets, in the regulation of muscle development.
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影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
3.7
作者：
Cardinali B;Castellani L;Fasanaro P;Basso A;Alemà S;Martelli F;Falcone G
通讯作者：
Falcone G
影响因子：
2.7
作者：
Biggar, Kyle K.;Dubuc, Adrian;Storey, Kenneth
通讯作者：
Storey, Kenneth
影响因子：
9.2
作者：
Caygill, Elizabeth E.;Johnston, Laura A.
通讯作者：
Johnston, Laura A.
影响因子：
30.8
作者：
Chen, JF;Mandel, EM;Wang, DZ
通讯作者：
Wang, DZ