IMMUNOHISTOCHEMICAL STUDY OF THE 4TH CELL TYPE IN THE OLFACTORY EPITHELIUM IN GUINEA-PIGS AND IN A PATIENT
IMMUNOHISTOCHEMICAL STUDY OF THE 4TH CELL TYPE IN THE OLFACTORY EPITHELIUM IN GUINEA-PIGS AND IN A PATIENT
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DOI：
10.1159/000276268
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发表时间：
1992-03-01
期刊：
影响因子：
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通讯作者：
KUWANO, R
中科院分区：
文献类型：
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作者：
YAMAGISHI, M;NAKAMURA, H;KUWANO, R
哺乳动物嗅上皮由支持细胞、嗅感受器细胞、基底细胞和新近发现的第四种细胞组成。在这项研究中，我们使用免疫组织化学技术检查这第四种细胞类型。用抗Purkinje特异性斑点-35蛋白（S-35）、抗S-100蛋白（S-100）、抗神经元特异性烯醇化酶（NSE）、抗嗜铬粒蛋白A和抗突触素抗血清进行免疫染色。第四类细胞只与抗S-35抗血清发生免疫反应，与其他抗血清不发生免疫反应。另一方面，在人S-100中，在第四细胞类型中观察到免疫反应性，以及对S-35的弱反应性。NSE免疫反应仅见于豚鼠和人的嗅感受器细胞。根据这些结果，假定第四种细胞类型是不同于嗅觉感受器细胞的第二种化学感受器，因为S-35和S-100是Ca++结合蛋白。
The mammalian olfactory epithelium consists of supporting cells, olfactory receptor cells, basal cells and a fourth cell type, which has recently been discovered. In this study we examine this fourth cell type using immunohistochemical techniques. Anti-Purkinje-specific spot-35 protein (S-35), anti-S-100 protein (S-100), anti-neuron-specific enolase (NSE), antichromogranin A and antisynaptophysin antisera were used for the immunostaining. The fourth cell type immunoreacted only to anti-S-35 antiserum and did not react to other antisera in guinea pigs. On the other hand, in the human S-100 immunoreactivity was seen in the fourth cell type as well as weak reactivity to S-35. NSE immunoreactivity was found only in the olfactory receptor cells of guinea pigs and the human. From these results it is assumed that this fourth cell type is a second chemoreceptor different from the olfactory receptor cells because S-35 and S-100 are Ca++-binding proteins.