Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster
Neuropeptide F immunoreactive clock neurons modify evening locomotor activity and free-running period in Drosophila melanogaster
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DOI：
10.1002/cne.22742
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发表时间：
2012-04-01
影响因子：
2.5
通讯作者：
Helfrich-Foerster, Charlotte
中科院分区：
文献类型：
--
作者：
Hermann, Christiane;Yoshii, Taishi;Helfrich-Foerster, Charlotte
黑腹果蝇（Drosophilamelanogaster）生物钟神经元的不同亚群在日常运动节律中具有不同的功能，其神经递质组成也存在差异。我们研究了神经肽F（NPF）免疫反应性时钟神经元在果蝇节律性运动行为中的功能。除了先前已经描述的三个LNd之外，我们新鉴定了第五个s-LNv和I-LNv的子集为NPF阳性。然后，我们选择性地消融不同子集的NPF表达神经元使用npfGal 4靶向表达的细胞死亡基因头退化缺陷（HID）与cryGal 80和pdfGal 80的组合。通过对果蝇日常运动节律的分析，我们发现NPF阳性的时钟神经元，特别是第5个s-LNv和LNd，参与了恒定黑暗（DD）中自由活动期的控制和光暗（LD）周期中夜间活动的相位和幅度。此外，我们发现，NPF和色素分散因子（PDF）免疫反应神经元的同时消融在LD中具有相加作用，导致晚上高峰期，甚至更先进的相比，PDF消融苍蝇。我们还发现，这个更先进的傍晚高峰是额外的幅度降低。为了明确将观察到的表型归因于NPF的作用，我们使用RNA干扰（RNAi）将其与PDF结合敲除，并进一步表明NPF在控制果蝇夜间活动中的可能作用。神经学比较杂志520：970987，2012。(C)2011 Wiley Periodicals，Inc.
Different subsets of Drosophila melanogaster's clock neurons are characterized by their specific functions in daily locomotor rhythms and the differences in their neurotransmitter composition. We investigated the function of the neuropeptide F (NPF) immunoreactive clock neurons in the rhythmic locomotor behavior of adult flies. We newly identified the fifth s-LNv and a subset of the l-LNvs as NPF-positive in addition to the three LNds that have been described previously. We then selectively ablated different subsets of NPF-expressing neurons using npfGal4-targeted expression of the cell death gene head involution defective (hid) in combination with cryGal80 and pdfGal80. By analyzing daily locomotor rhythms in these flies, we show that the NPF-positive clock neuronsespecially the fifth s-LNv and the LNdsare involved in both the control of the free-running period in constant darkness (DD) and the phasing and amplitude of the evening activity in light-dark (LD) cycles. Furthermore, we show that the simultaneous ablation of NPF and pigment dispersing factor (PDF)-immunoreactive neurons has additive effects in LD, resulting in an evening peak phase that is even more advanced in comparison to PDF-ablated flies. We also found that this more advanced evening peak is additionally reduced in amplitude. To putatively assign the observed phenotypes to the action of NPF, we knocked it down in conjunction with PDF using RNA-interference (RNAi) and further suggest a possible role for NPF in the control of the flies' evening activity. J. Comp. Neurol. 520:970987, 2012. (C) 2011 Wiley Periodicals, Inc.