STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells
STAT4 is a target of the hematopoietic zinc-finger transcription factor Ikaros in T cells
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DOI：
10.1016/j.febslet.2005.07.018
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发表时间：
2005-08-15
期刊：
影响因子：
3.5
通讯作者：
Venkatesh, B
中科院分区：
文献类型：
--
作者：
Yap, WH;Yeoh, E;Venkatesh, B
STAT 4是响应IL-12而激活的转录因子，并且参与Th 1细胞发育。然而，控制STAT 4基因转录的分子机制尚不清楚。对人、小鼠和红鳍东方鲀Stat 4基因5'端侧翼区的序列比较表明，这三种鱼中均含有高频率的Ikaros（Ik）结合元件。然后，我们使用Jurkat T细胞研究了Ik结合元件在人STAT 4启动子中的作用。反式激活、电泳迁移率变动分析和RNA干扰介导的基因敲低实验表明，Ik参与了人T细胞中STAT 4的调节。(c)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
STAT4 is a transcription factor activated in response to IL-12, and is involved in Th1 cell development. The molecular mechanisms controlling the transcription of the STAT4 gene are however, unclear. Sequence comparison of the 5' flanking regions of human, mouse and pufferfish (Fugu rubripes) Stat4 genes revealed a high frequency of Ikaros (Ik) binding elements in all three species. We then investigated the role of Ik binding elements in the human STAT4 promoter using Jurkat T cells. Transactivation, electrophoretic mobility shift assay and RNA interference-mediated gene knockdown experiments revealed that Ik is involved in the regulation of STAT4 in human T cells. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.