Method for simultaneous detection of functionality and tomography
Method for simultaneous detection of functionality and tomography
复制标题
同时检测功能和断层扫描的方法
DOI：
10.1117/12.831566
复制
发表时间：
2009
期刊：
影响因子：
1.9
通讯作者：
F. Schweitzer
中科院分区：
文献类型：
--
作者：
D. Schweitzer;M. Klemm;M. Hammer;S. Jentsch;F. Schweitzer
在荧光寿命激光扫描检眼镜的临床应用中，发现眼部自发荧光的斜率呈阶梯状，若将拟合区间的左边界设置在首次荧光出现的时间通道上，则拟合荧光时会导致寿命错误。如果拟合区间的左边界设置在检测到的荧光的最大值附近，则至少对于荧光衰减达到更好的拟合。通过对阶梯状斜坡的出现进行分析，发现阶梯状斜坡的产生是由于晶体透镜荧光和眼底荧光出现时间的不同。通过参数tci对指数模型函数的扩展导致斜率和荧光衰减两者的最佳拟合。该新参数描述了源自不同层的荧光的出现时间的差异。考虑到层之间的折射率，可以确定它们之间的几何距离。以这种方式，可以通过相同的测量来确定功能信息（寿命）和几何信息（距离）。为了达到与OCT相当的几何分辨率，脉冲和时间分辨率需要在30 fs的量级。
During clinical application of the fluorescence lifetime laser scanner ophthalmoscope, a stepped slope of ocular autofluorescence was found. Fitting of fluorescence results in wrong lifetimes if the left border of the fitting interval is set at the time channel of first appearance of fluorescence. A better fit was reached at least for the fluorescence decay if the left border of the fitting interval is set near the maximum of detected fluorescence. Analysing the appearance of the stepped slope, its origination was found by the different appearance time of fluorescence from the crystalline lens and from the fundus fluorescence. The extension of the exponential model function by a parameter tci results in an optimal fit of both the slope and of the fluorescence decay. This new parameter describes differences in the appearance time of fluorescence originating from different layers. Taking into account the refractive index between layers, the geometrical distance between them can be determined. In this way, functional information (lifetimes) and geometrical information (distances) can be determined by the same measurement. To reach geometrical resolution comparable with OCT, pulses and time resolution are required in the order of 30 fs.