HPLC Separation of Triacylglycerol Positional Isomers on a Polymeric ODS Column
HPLC Separation of Triacylglycerol Positional Isomers on a Polymeric ODS Column
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在聚合 ODS 柱上 HPLC 分离三酰甘油位置异构体
DOI：
10.2116/analsci.24.865
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发表时间：
2008
影响因子：
1.6
通讯作者：
S. Wada
中科院分区：
文献类型：
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作者：
Ikuma Kuroda;T. Nagai;H. Mizobe;Nobuhito Yoshimura;N. Gotoh;S. Wada
采用ODS聚合物色谱柱和循环HPLC系统，对甘油三酯位置异构体（TAG PI）1，3-二油酰-2-棕榈酰甘油（OPO）和1，2-二油酰-3-棕榈酰外消旋甘油（OOP）进行了分离。为了研究用于TAG-PI对的拆分的ODS柱种类和柱温度，使OPO和OOP的混合物在三种不同的柱温度（40、25或10°C）下经受配备有非封端的聚合物、封端的单体、封端的中间体或非封端的单体ODS柱的HPLC系统。只有非封端的聚合物ODS柱实现了OPO和OOP的分离，并且最低柱温（10°C）显示了对它们的最佳分离。另一对TAG-PI，即1，3-二棕榈酰基-2-油酰基-甘油（POP）和1，2-二棕榈酰基-3-油酰基-外消旋-甘油（PPO）的混合物也在五种不同的柱温（40、32、25、17和10 ℃）下经受配备有非封端聚合物或单体ODS柱的系统。因此，在25°C下，POP和PPO也仅在非封端的聚合ODS柱上分离。然而，在10°C下没有出现明显的峰。这些结果表明，聚合ODS固定相具有识别TAG-PI对之间的结构差异的能力。此外，柱温是分离TAG-PI对的一个非常重要的因素，最佳温度与TAG-PI在移动的相中的溶解度有关。此外，循环HPLC系统提供了用于TAG-PI对的分离和分析的测量。
A polymeric ODS column was applied to the resolution of triacylglycerol positional isomers (TAG-PI), i.e. 1,3-dioleoyl-2-palmitoyl-glycerol (OPO) and 1,2-dioleoyl-3-palmitoyl- rac -glycerol (OOP), with a recycle HPLC system. To investigate the ODS column species and the column temperatures for the resolution of a TAG-PI pair, a mixture of OPO and OOP was subjected to an HPLC system equipped with a non-endcapped polymeric, endcapped monomeric, endcapped intermediate, or non-endcapped monomeric ODS column at three different column temperatures (40, 25, or 10°C). Only the non-endcapped polymeric ODS column achieved the separation of OPO and OOP, and the lowest column temperature (10°C) showed the best resolution for them. The other pair of TAG-PI, a mixture of 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) and 1,2-dipalmitoyl-3-oleoyl- rac -glycerol (PPO) was also subjected to the system equipped with a non-endcapped polymeric or monomeric ODS column at five different column temperatures (40, 32, 25, 17, and 10°C). Thus, POP and PPO were also separated on only the non-endcapped polymeric ODS column at 25°C. However, no clear peak appeared at 10°C. These results would indicate that the polymeric ODS stationary phase has an ability to recognize the structural differences between TAG-PI pairs. Also, the column temperature is a very important factor for separating the TAG-PI pair, and the optimal temperature would relate to the solubility of TAG-PI in the mobile phase. Furthermore, the recycle HPLC system provided measurements for the separation and analysis of TAG-PI pairs.
通过反相高效液相色谱对异构三酰甘油进行区域选择性分离：固定相和流动相效应
DOI：
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发表时间：
2006
期刊：
J. Sep. Sci 29
影响因子：
--
作者：
S. Momchilova;Y. Itabashi;B. Nikolova-Damyanova and A. Kuksis
通讯作者：
B. Nikolova-Damyanova and A. Kuksis