Collection and characterization of amniotic fluid from scheduled C-section deliveries
Collection and characterization of amniotic fluid from scheduled C-section deliveries
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DOI：
10.1007/s10561-016-9572-7
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发表时间：
2016-09-01
影响因子：
1.5
通讯作者：
Reems, Jo-Anna
中科院分区：
文献类型：
--
作者：
Pierce, Jan;Jacobson, Pam;Reems, Jo-Anna
羊水(AF)具有抗炎、抗微生物和再生等特性，在临床上具有广泛的应用前景。这项研究的目的是评估从足月妊娠采集房颤的可行性，并评估房颤的非细胞和细胞特性用于临床应用。从计划进行剖腹产的孕妇那里获得捐赠者的知情同意和病史，并在收集当天进行传染病检测。对AFS的总容量、液体化学、总蛋白和透明质酸(HA)水平进行评估。还通过定量抗体阵列、细胞含量和支持血管生成的能力来评估房颤。36名孕妇同意并通过了捐赠者的筛选，可以生下组织。成功地采集了17个个体的房颤。房颤体积中位数为70毫升(10~815毫升；n=17)。液体化学成分相似，但在HA水平和细胞因子谱方面有所不同。细胞因子阵列显示，在所测试的400种蛋白中，平均有304+/-A20存在于房颤中，其中大多数细胞因子与宿主防御有关。房颤支持血管生成。房颤的细胞类型以上皮样细胞为主，仅有少量淋巴样细胞。培养显示出高度增殖的贴壁细胞群，能够产生治疗剂量的间充质基质细胞(MSCs)。这些发现表明，大量的房颤通常是从足月出生的婴儿身上收集的。AF含有大量的生物活性蛋白，仅有少量的MSCs。在不同的AFS中存在的成分的差异，需要进一步的研究来确定它们与特定临床应用的相关性。
Amniotic fluid (AF) possesses anti-inflammatory, anti-microbial and regenerative properties that make it attractive for use in clinical applications. The goals of this study were to assess the feasibility of collecting AF from full-term pregnancies and to evaluate non-cellular and cellular properties of AF for clinical applications. Donor informed consent and medical histories were obtained from pregnant women scheduled for C-sections and infectious disease testing was performed the day of collection. AFs were evaluated for total volume, fluid chemistries, total protein, and hyaluronic acid (HA) levels. AF was also assessed with quantitative antibody arrays, cellular content and for an ability to support angiogenesis. Thirty-six pregnant women consented and passed donor screening to give birth tissue. AF was successfully collected from 17 individuals. Median AF volumes were 70 mL (range 10-815 mL; n = 17). Fluid chemistries were similar, but some differences were noted in HA levels and cytokine profiles. Cytokine arrays revealed that an average of 304 +/- A 20 of 400 proteins tested were present in AF with a majority of cytokines associated with host defense. AF supported angiogenesis. Epithelioid cells were the major cell type in AF with only a minor population of lymphoid cells. Cultures revealed a highly proliferative population of adherent cells capable of producing therapeutic doses of mesenchymal stromal cells (MSCs). These findings showed that significant volumes of AF were routinely collected from full-term births. AF contained a number of bioactive proteins and only a rare population of MSCs. Variations noted in components present in different AFs, warrant further investigations to determine their relevance for specific clinical applications.