Plasmon resonance spectroscopy: probing molecular interactions at surfaces and interfaces
Plasmon resonance spectroscopy: probing molecular interactions at surfaces and interfaces
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DOI：
10.1155/2001/907405
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发表时间：
2001-01-01
影响因子：
--
通讯作者：
Tollin, G
中科院分区：
文献类型：
--
作者：
Salamon, Z;Tollin, G
表面等离子体共振（SPR）光谱可以应用于多种界面系统。它涉及薄金属膜中电子振荡（等离子体）的偏振光激发。这些产生了一个表面上定位的逃生电磁场，可用于探测垂直于固定在表面的材料平面的光学特性。光谱取决于三个参数：折射率（N），吸收系数（K）和厚度（T）。麦克斯韦方程提供了这些属性和SPR光谱之间的分析关系，从而允许其评估。该方法的扩展，称为等离激元 - 波导共振（CPWR或PWR），能够表征垂直且与表面平面平行的膜特性。在PWR设备中，金属膜被介电涂层覆盖，该介电涂层充当光学放大器，为金属层提供保护，并具有允许各种分子固定策略的表面。 PWR光谱的异常狭窄线宽度提高了灵敏度和分辨率。将描述该技术在几个生物膜系统中的应用，以证明其观察在膜蛋白功能期间发生结合和结构事件的能力。
Surface plasmon resonance (SPR) spectroscopy can be applied to a wide variety of interfacial systems. It involves resonant excitation by polarized light of electronic oscillations (plasmons) in a thin metal film. These generate a surface-localized evanescent electromagnetic field that can be used to probe the optical properties perpendicular to the film plane of materials immobilized at the surface. Spectra depend on three parameters: refractive index (n), absorption coefficient (k) and thickness (t). Maxwell's equations provide an analytical relationship between these properties and SPR spectra, allowing their evaluation. An extension of this methodology, called coupled plasmon-waveguide resonance (CPWR or PWR), is able to characterize film properties both perpendicular and parallel to the surface plane. In a PWR device, the metal film is covered with a dielectric coating that acts as an optical amplifier, provides protection for the metal layer, and possesses a surface that allows various molecular immobilization strategies. The exceptionally narrow line widths of PWR spectra yield enhanced sensitivity and resolution. The application of this technology to several biomembrane systems will be described, demonstrating its ability to observe both binding and structural events occurring during membrane protein function.