Translational Regulation of Metabolic Dynamics during Effector-Triggered Immunity
Translational Regulation of Metabolic Dynamics during Effector-Triggered Immunity
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DOI：
10.1016/j.molp.2019.09.009
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发表时间：
2020-01-06
期刊：
影响因子：
27.5
通讯作者：
Dong, Xinnian
中科院分区：
文献类型：
--
作者：
Yoo, Heejin;Greene, George H.;Dong, Xinnian
最近的研究表明，当植物识别与微生物相关的分子模式时，全球翻译重编程是模式触发免疫中的一种早期激活事件。然而，翻译调节是否也发生在随后的免疫反应中，如效应器触发免疫(ETI)，目前还不完全清楚。在这项研究中，我们对RPS2介导的ETI激活进行了拟南芥全基因组核糖体分析，发现特定的基因组受到翻译调控，主要与转录协调。这些基因编码与芳香族氨基酸、苯丙素、骆驼蓬蛋白和鞘磷脂代谢有关的酶。遗传和生化分析证实了这些成分在ETI中的功能意义。我们的发现为植物免疫反应的不同翻译调控提供了新的见解，并证明了代谢基因表达的翻译协调是ETI的重要策略。
Recent studies have shown that global translational reprogramming is an early activation event in pattern-triggered immunity, when plants recognize microbe-associated molecular patterns. However, it is not fully known whether translational regulation also occurs in subsequent immune responses, such as effector-triggered immunity (ETI). In this study, we performed genome-wide ribosome profiling in Arabidopsis upon RPS2-mediated ETI activation and discovered that specific groups of genes were translationally regulated, mostly in coordination with transcription. These genes encode enzymes involved in aromatic amino acid, phenylpropanoid, camalexin, and sphingolipid metabolism. The functional significance of these components in ETI was confirmed by genetic and biochemical analyses. Our findings provide new insights into diverse translational regulation of plant immune responses and demonstrate that translational coordination of metabolic gene expression is an important strategy for ETI.