Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS
Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS
复制标题
DOI：
10.1007/978-1-4939-7819-9_15
复制
发表时间：
2018-01-01
期刊：
影响因子：
--
通讯作者：
Dias, Daniel Anthony
中科院分区：
文献类型：
--
作者：
Jayasinghe, Nirupama Samanmalie;Mendis, Himasha;Dias, Daniel Anthony
气相色谱与三重四极杆质谱联用（GC-QqQ-MS）可用于准确定量从生物样品（如植物和包括血清和尿液在内的人体体液）中提取的内源性小分子。为了定量通常来自中心碳代谢的初级代谢物，例如来自糖酵解和戊糖磷酸途径的糖;以及参与三羧酸（TCA）循环的有机酸;必须从感兴趣的样品中提取极性内源性代谢物，化学衍生化并根据线性校准曲线定量至相应的真实标准。本章介绍了如何使用稳健、优化、基于多反应监测（MRM）的GC-QqQ-MS方法定量属于糖、糖醇、糖酸、糖磷酸盐和有机酸类的48种主要代谢物的组合。
Gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ-MS) can be used to accurately quantify endogenous small molecules extracted from biological samples such as plants and human fluids including sera and urine. In order to quantify primary metabolites typically from central carbon metabolism such as sugars from glycolysis and the pentose phosphate pathway; and organic acids involved in the tricarboxylic acid (TCA) cycle; polar endogenous metabolites must be extracted from the samples of interest, chemically derivatized and quantified against a linear calibration curve to a corresponding authentic standard. This chapter describes how to quantify a combination of 48 primary metabolites belonging to classes of sugars, sugar alcohols, sugar acids, sugar phosphates, and organic acids using a robust, optimized, multiple reaction monitoring (MRM)-based GC-QqQ-MS method.