An increased incidence of Propionibacterium acnes biofilms in acne vulgaris: a case-control study
An increased incidence of Propionibacterium acnes biofilms in acne vulgaris: a case-control study
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DOI：
10.1111/j.1365-2133.2012.10897.x
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发表时间：
2012-07-01
影响因子：
10.3
通讯作者：
Alexeyev, O. A.
中科院分区：
文献类型：
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作者：
Jahns, A. C.;Lundskog, B.;Alexeyev, O. A.
寻常痤疮是一种皮脂囊疾病。痤疮丙酸杆菌可参与炎症性痤疮。目的本病例对照研究旨在调查痤疮面部活检中痤疮的发生和定位，并表征痤疮痤疮在皮肤隔室的类型。方法应用特异性单克隆和多克隆抗体对38例痤疮患者及对照者进行皮肤活检，确定痤疮P.的定位和特征，并检测推测的毒力决定因素共溶血素CAMP因子的表达。结果18例（47%）痤疮患者和8例（21%）对照样本中存在毛囊性痤疮[比值比（OR） 3.37, 95%可信区间（CI） 1.23 ~ 9.23；P = 0.017]。在14例（37%）痤疮患者的样本中，痤疮假单胞杆菌在皮脂腺毛囊的大菌落/生物膜中可见，而只有5例（13%）对照样本（OR 3.85, 95% CI 1.22-12.14; P = 0.021）。在病例和对照样本中均检测到由表达CAMP1的混合痤疮假单胞菌种型组成的大菌落/生物膜。只有4个样品的葡萄球菌和真菌检测呈阳性，没有观察到。结论我们首次在皮肤活检的皮脂腺毛囊的大菌落/生物膜中观察到不同的痤疮假单胞菌种型。我们的研究结果支持痤疮假单胞菌在痤疮发病机制中发挥作用的假设，因为痤疮样本显示，痤疮假单胞菌在卵泡中定植的患病率更高，无论是在含有痤疮假单胞菌的卵泡中，还是在大菌落/生物膜中，细菌数量都比对照样本多。
Background Acne vulgaris is a disorder of the sebaceous follicles. Propionibacterium acnes can be involved in inflammatory acne.Objectives This case-control study aimed at investigating the occurrence and localization of P. acnes in facial biopsies in acne and to characterize the P. acnes phylotype in skin compartments.Methods Specific monoclonal and polyclonal antibodies were applied to skin biopsies of 38 patients with acne and matching controls to localize and characterize P. acnes and to determine expression of co-haemolysin CAMP factor, a putative virulence determinant.Results Follicular P. acnes was demonstrated in 18 (47%) samples from patients with acne and eight (21%) control samples [odds ratio (OR) 3.37, 95% confidence interval (CI) 1.23-9.23; P = 0.017]. In 14 (37%) samples from patients with acne, P. acnes was visualized in large macrocolonies/biofilms in sebaceous follicles compared with only five (13%) control samples (OR 3.85, 95% CI 1.22-12.14; P = 0.021). Macrocolonies/biofilms consisting of mixed P. acnes phylotypes expressing CAMP1 were detected in both case and control samples. Only four samples tested positive for the presence of Staphylococcus spp. and fungi were not observed.Conclusions We have for the first time visualized different P. acnes phylotypes in macrocolonies/biofilms in sebaceous follicles of skin biopsies. Our results support the hypothesis that P. acnes can play a role in the pathogenesis of acne as acne samples showed a higher prevalence of follicular P. acnes colonization, both in terms of follicles containing P. acnes and the greater numbers of bacteria in macrocolonies/biofilms than in control samples.