Gamma interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65
Gamma interferon expression in CD8+ T cells is a marker for circulating cytotoxic T lymphocytes that recognize an HLA A2-restricted epitope of human cytomegalovirus phosphoprotein pp65
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DOI：
10.1128/cdli.8.3.628-631.2001
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发表时间：
2001-05-01
期刊：
影响因子：
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通讯作者：
Maino, VC
中科院分区：
文献类型：
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作者：
Ghanekar, SA;Nomura, LE;Maino, VC
具有细胞毒活性的抗原特异性CD8(+)T细胞在对感染性病原体的免疫反应中通常是至关重要的。为了确定细胞毒性T淋巴细胞(CTL)是否可以替代干扰素的表达，对CD8(+)与人巨细胞病毒特异性CTL反应进行了相关研究。细胞因子BOW细胞术检测T细胞干扰素-γ的表达，在Cr-51释放实验中，多肽脉冲靶标的特异性裂解与6h或7d多肽激活的CD8(+)T细胞表达干扰素-γ的频率呈显著正相关(r(2)=0.72或r(2)=0.91)。对表达穿孔素的反应细胞的计数并没有改善这种相关性。穿孔素是另一种与CTL相关的标记。这些结果表明，干扰素-γ的表达可以作为CTL前体细胞鉴定的功能替代指标。
Antigen-specific CD8(+) T cells with cytotoxic activity are often critical in immune responses to infectious pathogens. To determine whether gamma interferon (IFN-gamma) expression is a surrogate marker for cytotoxic T lymphocytes (CTL), human cytomegalovirus-specific CTL responses were correlated with CD8(+). T-cell IFN-gamma expression determined by cytokine Bow cytometry, A strong positive correlation was observed between specific lysis of peptide-pulsed targets in a Cr-51 release assay and frequencies of peptide-activated CD8(+) T cells expressing IFN-gamma at 6 h (r(2) = 0.72) or 7 days (r(2) = 0.91). Enumeration of responding cells expressing perforin, another marker associated with CTL, did not improve this correlation. These results demonstrate that IFN-gamma expression can be a functional surrogate for identification of CTL precursor cells.